Detailed information of TA system TA162892

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 841626..842280
• Replicon: chromosome
• Accession: NZ_CP056180
• Organism: Citrobacter sp. RHBSTW-01044

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV352_RS04115
• Protein ID: WP_016154348.1
• Coordinates: 841873..842280 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV352_RS04110
• Protein ID: WP_016154349.1
• Coordinates: 841626..841892 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV352_RS04085	836839..838272	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV352_RS04090	838393..839121	-	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
HV352_RS04095	839174..839485	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV352_RS04100	839649..840308	+	660	WP_016154351.1	hemolysin III family protein	-
HV352_RS04105	840388..841368	-	981	WP_016154350.1	tRNA-modifying protein YgfZ	-
HV352_RS04110	841626..841892	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV352_RS04115	841873..842280	+	408	WP_016154348.1	protein YgfX	Toxin
HV352_RS04120	842381..842902	-	522	WP_016154347.1	flavodoxin FldB	-
HV352_RS04125	843016..843912	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV352_RS04130	843936..844649	+	714	WP_016154345.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV352_RS04135	844655..846388	+	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T162892 WP_016154348.1 NZ_CP056180:841873-842280 [Citrobacter sp. RHBSTW-01044]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T162892 NZ_CP072156:c2958217-2957954 [Rhizobium sp. B230/85]
ATGGCTTTTGAGTTTGATCCCGACAAAAGCAGAACAAACAAAATCAAGCACGGTATTGATCTTGTGGAGGCGCAGCGTCT
TTGGATCGATCGCTTTTTGATTTCATTCCCCGCTCGGGGCGGACTTGAAAGCCGGTTTGTCGCGGTCGGTCAAATAGACG
GGAAGCATTGGTCCGCGATTTGCACGAGACGTCATGACAATATCAGGATCATTTCGGTTCGCAGGTCGAGGAAAGAGGAG
ATAGCTCTCTATGAAGGCAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT162892 WP_016154349.1 NZ_CP056180:841626-841892 [Citrobacter sp. RHBSTW-01044]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT162892 NZ_CP072156:c2957967-2957740 [Rhizobium sp. B230/85]
ATGAAGGCAAGTGAACTCGACGAGAAATTCGACAATGGCGAGGATATACTCGACTATCTCGACCTCACCAAGGCAACCCG
CCCAAACATGGCGACCCAAAAGGTCAGCGTGGATTTTGCAACCTCAGTGGTCAATAGCCTCGACGAGGAAGCAGCCCGAC
TCGGGCTGACACGGCAATCACTGATCAATTTGTGGATCGAAGAGCGTCTGTCCGCCGCTCAGGATTAG

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6