Detailed information of TA system TA162890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2492..3135
• Replicon: plasmid pRHBSTW-00695_3
• Accession: NZ_CP056170
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV268_RS24350
• Protein ID: WP_016236302.1
• Coordinates: 2492..2908 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HV268_RS24355
• Protein ID: WP_001261282.1
• Coordinates: 2905..3135 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS24335	193..483	-	291	WP_001247892.1	nucleotidyltransferase	-
HV268_RS24340	620..1324	+	705	WP_023327680.1	IS6-like element IS26 family transposase	-
HV268_RS24345	1375..2418	-	1044	Protein_3	AAA family ATPase	-
HV268_RS24350	2492..2908	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV268_RS24355	2905..3135	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV268_RS24360	3092..3304	+	213	Protein_6	hypothetical protein	-
HV268_RS24365	3619..3933	+	315	WP_096152151.1	hypothetical protein	-
HV268_RS24370	4028..4357	+	330	WP_096151727.1	hypothetical protein	-
HV268_RS24375	4406..5224	+	819	WP_096149273.1	hypothetical protein	-
HV268_RS24380	5224..5997	+	774	WP_008502207.1	site-specific integrase	-
HV268_RS24385	6074..6379	-	306	WP_096152150.1	hypothetical protein	-
HV268_RS24390	6376..7002	-	627	WP_008502208.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26712	26712
	-	flank	IS/Tn	-	-	620..1324	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T162890 WP_016236302.1 NZ_CP056170:c2908-2492 [Enterobacter roggenkampii]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T162890 NZ_CP072156:c2863051-2862749 [Rhizobium sp. B230/85]
TTGCCAGTGAAGACGCTACCTTTCGATGCTGCAGATCATTTGCAGAGCGGCCAATCCCAGCTTGAGTTGCTGAGCGATGC
TTTTAATTCCGGGAGCGCATCCTATGTCGCGGTGGCGTTGGGCGTCATTGCGCGCGCCAAAGGCATGACGGAAGTATCGA
AAACGGCAGGCATCACCCGCGAAGCGCTATACAAAGCTCTCAGCGCCGACGGCGATCCGAAGCTGAGCACGCTGCTTGGC
GTGCTCAACGCTCTCGACCTCCAGCTGAAATTGGCCCCTGGCAACAAAAAAGCCCCGGACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT162890 WP_001261282.1 NZ_CP056170:c3135-2905 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT162890 NZ_CP072156:c2863345-2863052 [Rhizobium sp. B230/85]
ATGCCCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTTGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGAATGATTTGGAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3