Detailed information of TA system TA162889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 84198..84937
• Replicon: plasmid pRHBSTW-00695_2
• Accession: NZ_CP056169
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: HV268_RS23715
• Protein ID: WP_013087172.1
• Coordinates: 84452..84937 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: HV268_RS23710
• Protein ID: WP_012540086.1
• Coordinates: 84198..84464 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS23680	79824..80207	+	384	WP_001201739.1	IS66 family insertion sequence hypothetical protein	-
HV268_RS23685	80204..80551	+	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-
HV268_RS23690	80601..82136	+	1536	WP_001000409.1	IS66 family transposase	-
HV268_RS23695	82306..82851	-	546	WP_013087169.1	sigma-70 family RNA polymerase sigma factor	-
HV268_RS23700	82860..83573	-	714	Protein_90	arsenical resistance protein ArsH	-
HV268_RS23705	83575..83898	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
HV268_RS23710	84198..84464	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
HV268_RS23715	84452..84937	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
HV268_RS23720	85355..86695	+	1341	WP_181531663.1	ISNCY family transposase	-
HV268_RS23725	86951..87373	-	423	WP_032072095.1	nickel resistance OB fold protein NcrY	-
HV268_RS23730	87535..88665	-	1131	WP_004196366.1	Ni(II)/Co(II) efflux transporter permease subunit NcrC	-
HV268_RS23735	88678..88947	-	270	WP_004196355.1	nickel-sensing transcriptional repressor NcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkF / mrkD / mrkC / mrkB / mrkA	1..197969	197969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T162889 WP_013087172.1 NZ_CP056169:84452-84937 [Enterobacter roggenkampii]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T162889 NZ_CP072156:1479844-1480089 [Rhizobium sp. B230/85]
ATGAAGGACGTTGTCGTATCCCGACTTGCCGACAAAGCACTTATGCGCATGCAGCCAAAACGGCGGCTGGCGATTATTGA
AAAAGTCAAAGCCTATGCGCGCGGAGAGATTGTCGACATCAAGAAGATGAAAGGCGGAAATCTCTACCGTATCAGGGTCG
GACAGGATCGCGTCATCATCGACGACAAGGGTAGGATTGTCGTGGTCATCGATGCCGGTCCGCGGGGCAGTATCTACAAG
GAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT162889 WP_012540086.1 NZ_CP056169:84198-84464 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT162889 NZ_CP072156:1480096-1480458 [Rhizobium sp. B230/85]
ATGGGCGATATCAAGAGGTTCGAAATCGACGGCAAACCCTATGTGCTGTTGAGTGAAGAGGATTATGAGGATCTCGTCGA
TGGACTGAGGGCGAATGCTGTCCTGGCCGGGATTTCTGCAGGAGAAGAAACCTGGCCCTTGGAGATCGTTGAGGCGCGTG
CCAATGGCGGAAATGCCGTTCGTATCTTCCGAAACTATCGCCGCATGACTGTTACCGAACTTGCCACGGCCGCCGGAATT
TCGCAGCCCTATCTCTCTGAGATCGAGGCAGGCAAAAAAACCGGCAGTGTCGATGTCCTGAAGCGTATCGCTAACGCCCT
CAAGGTCGATCTCGACGATCTCGTCGTCAACGTGGCGGACTGA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8