Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4092543..4093119 Replicon chromosome
Accession NZ_CP056168
Organism Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag HV268_RS20025 Protein ID WP_025912099.1
Coordinates 4092832..4093119 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag HV268_RS20020 Protein ID WP_023293359.1
Coordinates 4092543..4092845 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV268_RS19995 4088653..4089198 + 546 WP_008501313.1 porin -
HV268_RS20000 4089198..4089518 + 321 WP_008501314.1 hypothetical protein -
HV268_RS20005 4089561..4090931 - 1371 WP_181530820.1 NAD-dependent succinate-semialdehyde dehydrogenase -
HV268_RS20010 4091083..4091826 + 744 WP_161622971.1 AraC family transcriptional regulator -
HV268_RS20015 4091874..4092512 + 639 WP_161622970.1 LysE family translocator -
HV268_RS20020 4092543..4092845 - 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
HV268_RS20025 4092832..4093119 - 288 WP_025912099.1 BrnT family toxin Toxin
HV268_RS20030 4093289..4094560 + 1272 WP_181530822.1 DUF445 domain-containing protein -
HV268_RS20035 4094557..4095633 - 1077 WP_045335580.1 DUF2955 domain-containing protein -
HV268_RS20040 4095623..4096690 - 1068 WP_025912094.1 HlyD family secretion protein -
HV268_RS20045 4096687..4097157 - 471 WP_008501325.1 MarR family transcriptional regulator -
HV268_RS20050 4097304..4097999 - 696 WP_045335574.1 winged helix-turn-helix domain-containing protein -
HV268_RS20055 4097992..4098108 - 117 Protein_3924 amino acid-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T162887 WP_025912099.1 NZ_CP056168:c4093119-4092832 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T162887 NZ_CP072156:1294244-1294528 [Rhizobium sp. B230/85]
ATGGTAATTCTTTCGTTCAAGGATGGGTCGACGCGCGCCGTTGCTTCCGGCGTTGTGCCGAAAGGCTTTCCCGCCGACCT
TGCGCGACGTGCTATTCGCAAGCTGACCATGATCGAATATGCCAACCAGCTTTCGGACCTACGTTCGCCGCCCGCAAATC
ATCTCGAGGCGTTGAAGGGAGATCGTGCCGGCCAGCATTCCATCCGCATCAACGATCAATGGCGGATTTGCTTCGTCTGG
ACCGATGCTGGCGTCGAAGATGTCGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT162887 WP_023293359.1 NZ_CP056168:c4092845-4092543 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT162887 NZ_CP072156:1294539-1294835 [Rhizobium sp. B230/85]
ATGAAGCACATTCTCCCACCGGTGCATCCGGGTGAAATCCTTCGGGAGGAGTATCTGGCACCGCTTAAAATGAGCGCCGG
TGCCCTCGCGCGCAAGCTGAACGTCCCGCGAACGCGCATCGAGCGTCTGGTCGCCGAGGAAACGCCGGTGACCATCGATA
CCGCGTTGCGGTTGGCCCGCTTTTTCGACACGACGCCGGAGTTTTGGATGAATATGCAAACTGGCTACGAACTGAAGCGC
CAGGCGGAGGAAAAGAGAGCCGAGATCGCCGCTATCCCAGTCCTCCATGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References