Detailed information of TA system TA162886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3577591..3578211
• Replicon: chromosome
• Accession: NZ_CP056168
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: HV268_RS17465
• Protein ID: WP_008499287.1
• Coordinates: 3577993..3578211 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV268_RS17460
• Protein ID: WP_181530683.1
• Coordinates: 3577591..3577965 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS17450	3572719..3573912	+	1194	WP_021241633.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV268_RS17455	3573935..3577081	+	3147	WP_008499289.1	multidrug efflux RND transporter permease subunit	-
HV268_RS17460	3577591..3577965	+	375	WP_181530683.1	Hha toxicity modulator TomB	Antitoxin
HV268_RS17465	3577993..3578211	+	219	WP_008499287.1	hemolysin expression modulator Hha	Toxin
HV268_RS17470	3578418..3578969	+	552	WP_008499286.1	maltose O-acetyltransferase	-
HV268_RS17475	3579087..3579554	+	468	WP_008499285.1	membrane protein	-
HV268_RS17480	3579526..3580986	-	1461	WP_161622866.1	PLP-dependent aminotransferase family protein	-
HV268_RS17485	3581088..3581798	+	711	WP_181530684.1	GNAT family N-acetyltransferase	-
HV268_RS17490	3581795..3581935	-	141	WP_034458060.1	type B 50S ribosomal protein L36	-
HV268_RS17495	3581938..3582198	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T162886 WP_008499287.1 NZ_CP056168:3577993-3578211 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T162886 NZ_CP072156:c1220973-1220713 [Rhizobium sp. B230/85]
ATGAAAATTACGTGGGACGAGCGCAAGCGGCTCGCAAATTTCGACAAGCACGGCCTCGATTTCGCCGAACTCGACGCGGA
TTTCTTTCTTGCAGCTCTTTTGCAACCGGCAAAACTCGGACGGTTCAAGGCCATCGGGCGACGACATGACGGTACGACCG
CAGTCATCTTCGCCGTTCTCGGGCGCGAGGCGGTCTCGGTAATATCGATGCGGCCTGCGCACAAAGCCGAAAGGAGCCTG
TTGAATGACAAAGAAAACTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14481.32 Da
• Isoelectric Point: 5.1625

>AT162886 WP_181530683.1 NZ_CP056168:3577591-3577965 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAKDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT162886 NZ_CP072156:c1220729-1220421 [Rhizobium sp. B230/85]
ATGACAAAGAAAACTGAAATGCGGAGCGACTTCCAGAATGGCCGGGGCTATTCAGACCAGGATTGGGAGGAGGTTTCCGA
CAATCCTGAATGGACCGAGGAAGACTTCAAGAATGCCCGTCCGTTTGCCGAGGTTTTCCCGGATCTGGCGGAAAGCATTC
GCCGGGCCCGGGGGCGACCGGCGGTCGAGGCGCCGAAGCGGCAAATCTCGCTGCGGCTCGATCCCGATGTCATCGATGCC
TTCAAGGCCACCGGCAAGGGCTGGCAGAGCCGTATCAACGAGGCTTTGCGCAAGGCGGCCAAAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38