Detailed information of TA system TA162884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2983793..2984590
• Replicon: chromosome
• Accession: NZ_CP056168
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A155TD66
• Locus tag: HV268_RS14675
• Protein ID: WP_032651255.1
• Coordinates: 2983793..2984314 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HV268_RS14680
• Protein ID: WP_123922612.1
• Coordinates: 2984321..2984590 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS14645	2979376..2980065	+	690	WP_123922608.1	pyrimidine utilization protein B	-
HV268_RS14650	2980077..2980463	+	387	WP_008499822.1	pyrimidine utilization protein C	-
HV268_RS14655	2980471..2981271	+	801	WP_181530519.1	pyrimidine utilization protein D	-
HV268_RS14660	2981281..2981871	+	591	WP_023327079.1	malonic semialdehyde reductase	-
HV268_RS14665	2981881..2982375	+	495	WP_123922610.1	pyrimidine utilization flavin reductase protein F	-
HV268_RS14670	2982397..2983719	+	1323	WP_008499826.1	pyrimidine utilization transport protein G	-
HV268_RS14675	2983793..2984314	-	522	WP_032651255.1	GNAT family N-acetyltransferase	Toxin
HV268_RS14680	2984321..2984590	-	270	WP_123922612.1	DUF1778 domain-containing protein	Antitoxin
HV268_RS14685	2984652..2985569	-	918	WP_181530521.1	DMT family transporter	-
HV268_RS14690	2985688..2985858	-	171	WP_013097201.1	general stress protein	-
HV268_RS14695	2986250..2986846	+	597	WP_181530523.1	NAD(P)H:quinone oxidoreductase	-
HV268_RS14700	2986867..2987094	+	228	WP_008499832.1	YccJ family protein	-
HV268_RS14705	2987130..2988371	-	1242	WP_008499833.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
HV268_RS14710	2988524..2988967	+	444	WP_008499834.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19714.46 Da
• Isoelectric Point: 7.0497

>T162884 WP_032651255.1 NZ_CP056168:c2984314-2983793 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T162884 NZ_CP072152:c1615541-1615158 [Rhizobium sp. K15/93]
GTGATATTGGTAGACACCTCGATCTGGATTGATCATTTCCGCCATGGTGACGTGGAGTTGCGCAAAGTTATCGCGGACGA
CCGGCTACTCTGTCACCCCGTCGTTGTCGGAGAATTGGCGCTCGGCAGTCTTCGCGACCGTGGGGCCGTCATGGCGTTTT
TTTCGGCGCAGAGAGAGGCACTAGTCGCTAGCCATTACGAAGTAATAACGATGATCGGTCGGCACTCGATTTTCAGCATG
GGGATCGGGTACACGGATGCACATTTGCTGGCTTCGATACTGCTCGATAGGCGATCGTTGCTATGGACCAGAGACAAGCG
TTTAGCTGCCGCGGCACGAAAAGCGGGTGCCTCACTGTATGCACCCACGAGCGCGCCAAACTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10276.75 Da
• Isoelectric Point: 5.8900

>AT162884 WP_123922612.1 NZ_CP056168:c2984590-2984321 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIENPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT162884 NZ_CP072152:c1615738-1615538 [Rhizobium sp. K15/93]
ATGCGATCCACTATCAATCTCGATGATAACCTCATGGAACAGGCGAGGTCCCTGACTGGAACAAAGGAAACAGCCGCACT
TGTCCGCCAGGCCTTGGAGACACTTGTGCGGGTCGAATCCGGAAAGCGTCTCGCTGCTCTCGGGGGTACCATGCCAGATG
CAGAGGCAGCCCCTCGCCGCCGGAGCGAAGCGGCGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A155TD66