Detailed information of TA system TA162875

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 811054..811711
• Replicon: chromosome
• Accession: NZ_CP056168
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W7P4L3
• Locus tag: HV268_RS03755
• Protein ID: WP_023326024.1
• Coordinates: 811301..811711 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W7NX65
• Locus tag: HV268_RS03750
• Protein ID: WP_006178375.1
• Coordinates: 811054..811320 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS03730	806488..807921	-	1434	WP_023294510.1	6-phospho-beta-glucosidase BglA	-
HV268_RS03735	808038..808769	-	732	WP_021242053.1	MurR/RpiR family transcriptional regulator	-
HV268_RS03740	809035..809694	+	660	WP_045335239.1	hemolysin III family protein	-
HV268_RS03745	809779..810759	-	981	WP_100122211.1	tRNA-modifying protein YgfZ	-
HV268_RS03750	811054..811320	+	267	WP_006178375.1	FAD assembly factor SdhE	Antitoxin
HV268_RS03755	811301..811711	+	411	WP_023326024.1	protein YgfX	Toxin
HV268_RS03760	811718..812239	-	522	WP_045909912.1	flavodoxin FldB	-
HV268_RS03765	812341..813237	+	897	WP_181531124.1	site-specific tyrosine recombinase XerD	-
HV268_RS03770	813266..813979	+	714	WP_008499708.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV268_RS03775	813985..815718	+	1734	WP_055322108.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16063.06 Da
• Isoelectric Point: 10.9468

>T162875 WP_023326024.1 NZ_CP056168:811301-811711 [Enterobacter roggenkampii]
VVLWQSDLRVSWRSQWMSLLLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNCGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T162875 NZ_CP072151:518356-518658 [Rhizobium sp. K15/93]
TTGCCAGTGAAGACGCTACCTTTCGATGCTGCAGATCATTTGCAGAGCGGCCAATCCCAGCTTGAGTTGCTGAGCGATGC
TTTTAATTCCGGGAGCGCATCCTATGTCGCGGTGGCGTTGGGCGTCATTGCGCGCGCCAAAGGCATGACGGAAGTATCGA
AAACGGCAGGCATCACCCGCGAAGCGCTATACAAAGCTCTCAGCGCCGACGGCGATCCGAAGCTGAGCACGCTGCTTGGC
GTGCTCAACGCTCTCGACCTCCAGCTGAAATTGGCCCCTGGCAACAAAAAAGCCCCGGACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1340

>AT162875 WP_006178375.1 NZ_CP056168:811054-811320 [Enterobacter roggenkampii]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT162875 NZ_CP072151:518062-518355 [Rhizobium sp. K15/93]
ATGCCCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTTGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGAATGATTTGGAGATTTGA

Structures

Toxin

• AlphaFold DB: W7P4L3

Antitoxin

• AlphaFold DB: W7NX65