Detailed information of TA system TA162861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2315732..2317320
• Replicon: chromosome
• Accession: NZ_CP056165
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A7U9DLY5
• Locus tag: HV276_RS11100
• Protein ID: WP_001125477.1
• Coordinates: 2315998..2317320 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HV276_RS11095
• Protein ID: WP_001516368.1
• Coordinates: 2315732..2315998 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS11080	2312408..2313322	+	915	WP_001516366.1	bestrophin family protein	-
HV276_RS11085	2313326..2314084	-	759	WP_061090837.1	trans-aconitate 2-methyltransferase	-
HV276_RS11090	2314512..2315468	+	957	WP_000197006.1	hypothetical protein	-
HV276_RS11095	2315732..2315998	+	267	WP_001516368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HV276_RS11100	2315998..2317320	+	1323	WP_001125477.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HV276_RS11105	2317538..2317657	-	120	WP_100756432.1	nonstructural protein	-
HV276_RS11110	2317659..2318444	-	786	WP_181503318.1	hypothetical protein	-
HV276_RS11115	2318499..2320217	-	1719	WP_181503319.1	phage capsid protein	-
HV276_RS11120	2320363..2320797	-	435	WP_181503320.1	hypothetical protein	-
HV276_RS11125	2320856..2321134	-	279	WP_181503321.1	hypothetical protein	-
HV276_RS11130	2321121..2321984	-	864	WP_181503322.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2315732..2332252	16520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49556.21 Da
• Isoelectric Point: 8.5841

>T162861 WP_001125477.1 NZ_CP056165:2315998-2317320 [Escherichia marmotae]
MPKLVTWMNNQRVGELTKLTNGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPEDETVTRPIMVWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRMGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKEFGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGSTDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGRKTEIDKIYPRHFLATAKALKFPEVQMHEILSDFARMIP
AALDNVKASLPADFPENVLTAIETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T162861 NZ_CP072146:55834-56106 [Rhizobium sp. K1/93]
ATGGCGTGGAGGATTGAGTTTCAGCGGCCGGCGGAACGCGATCTTGAAAAGCTCGATCATGAGAGCGTGAAAAGGATACT
CCGTTTCCTTCACACGCGTGTCGCCAGCCTCGAGGACCCCCGGTCGATAGGGGAGGCGCTTCGCGGATCGGAACTTGGAA
ACTTCTGGAAGTATCGTGTTGGAGACTATCGCATCATTGCCGACATAGACGACGGCACCGTTCGGATATTGGTCGTCCGC
ATTGGCAATCGGAAGGACGTCTATCGCCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10034.37 Da
• Isoelectric Point: 4.5668

>AT162861 WP_001516368.1 NZ_CP056165:2315732-2315998 [Escherichia marmotae]
MMSFPMIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLNLSMTLCDTKNDSPES
TDQQDLEW

Nucleotide

• Length: 267 bp

>AT162861 NZ_CP072146:55626-55850 [Rhizobium sp. K1/93]
ATGCTTGCCCTGCGTTTGCCTCCTGACATTGAAGCAAGACTAGATGAGCTCGCCAAACGCACCGGGCGGAGCAAGAGCTT
CTATGCGCGTCAAGCGATATTGGAACACTTGGACGATCTGGAAGACCTCTACGTCGCCGAGCAGATGCTCGAAGAATTCC
GTAGAGGCGACAGTGATAGCATTTCTCTCGCTGAGTTGATGGCGCGCCATGGCGTGGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DLY5