Detailed information of TA system TA162850

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1034819..1035497
• Replicon: chromosome
• Accession: NZ_CP056165
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV276_RS04905
• Protein ID: WP_115775630.1
• Coordinates: 1034819..1035160 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A7U9DI50
• Locus tag: HV276_RS04910
• Protein ID: WP_016248357.1
• Coordinates: 1035180..1035497 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS04885	1030189..1030866	+	678	WP_001240678.1	hypothetical protein	-
HV276_RS04890	1030947..1032224	-	1278	WP_001269640.1	tyrosine-type recombinase/integrase	-
HV276_RS04895	1032711..1033370	+	660	WP_016248354.1	HNH endonuclease	-
HV276_RS04900	1033349..1034419	-	1071	WP_016248355.1	DNA cytosine methyltransferase	-
HV276_RS04905	1034819..1035160	-	342	WP_115775630.1	TA system toxin CbtA family protein	Toxin
HV276_RS04910	1035180..1035497	-	318	WP_016248357.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV276_RS04915	1035516..1035737	-	222	WP_016248358.1	DUF987 domain-containing protein	-
HV276_RS04920	1035746..1036222	-	477	WP_016248359.1	RadC family protein	-
HV276_RS04925	1036238..1036696	-	459	WP_016248360.1	antirestriction protein	-
HV276_RS04930	1036782..1037018	-	237	WP_016248361.1	DUF905 domain-containing protein	-
HV276_RS04935	1037096..1037506	-	411	WP_115775632.1	hypothetical protein	-
HV276_RS04940	1037573..1038010	-	438	WP_038434461.1	hypothetical protein	-
HV276_RS04945	1038052..1038588	-	537	WP_016248365.1	DUF4339 domain-containing protein	-
HV276_RS04950	1038614..1039324	-	711	WP_097569200.1	DeoR family transcriptional regulator	-
HV276_RS04955	1039533..1040357	-	825	WP_181503202.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1011291..1071850	60559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12805.81 Da
• Isoelectric Point: 9.7746

>T162850 WP_115775630.1 NZ_CP056165:c1035160-1034819 [Escherichia marmotae]
MKTLPATISRAAKPCLSPVGVWQMLLTRLLEQHYGLTLNDTPFSDKTVIKEHIDAGITLADAVNFLVDKYELVRIDRRGF
YYREQSPYLRAVDILRARQATGLLRRSQSDLGG

Nucleotide

• Length: 342 bp

>T162850 NZ_CP072141:2679723-2680028 [Rhizobium sp. L51/94]
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTAGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGACGGTAACCTGCGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGTTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACGCAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCCGAATGGAAGGAGCGGAACCAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11888.50 Da
• Isoelectric Point: 6.9464

>AT162850 WP_016248357.1 NZ_CP056165:c1035497-1035180 [Escherichia marmotae]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASIIGKFSDAECRKLDETFPHFISQMELMLITGELNPRHAHCVNLYH
NGFTCEADALGSCGYAYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT162850 NZ_CP072141:2680025-2680327 [Rhizobium sp. L51/94]
ATGACCTCAGACACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAGCGAGAGTGCAATCGATGAATTTCTCGCTGCGGC
ATTCGAAACCGAGGACCCGGCATTTATCGCCAAAAGTCTTGGGGTGATTGCCAAAACCCGAAACATGAGCGCGATCGCCC
GAGATGTTGGAATGAGCCGTGCTGCCCTCTACAAATCGTTGAGCGGCAAAGGCAATCCGGAATTTGCGACCATTATAAAG
GTTATGAAGGCACTTGGCCTCGGCCTGGCTCCTGTCTCTCGGTCTAACCAGGGCGCTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DI50