Detailed information of TA system TA162842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 32909..33444
• Replicon: plasmid pRHBSTW-00814_2
• Accession: NZ_CP056160
• Organism: Escherichia marmotae strain RHBSTW-00814

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV284_RS24205
• Protein ID: WP_134901167.1
• Coordinates: 33157..33444 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: HV284_RS24200
• Protein ID: WP_001132900.1
• Coordinates: 32909..33160 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV284_RS24185	28499..29023	-	525	WP_181475208.1	type 1 fimbrial protein	-
HV284_RS24190	29269..29577	-	309	WP_181475198.1	adhesin biosynthesis transcription regulatory family protein	-
HV284_RS24195	30186..32634	-	2449	Protein_39	Tn3 family transposase	-
HV284_RS24200	32909..33160	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV284_RS24205	33157..33444	+	288	WP_134901167.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV284_RS24210	33636..33755	+	120	Protein_42	helix-turn-helix transcriptional regulator	-
HV284_RS24215	33898..34449	-	552	WP_052980227.1	plasmid stability family protein	-
HV284_RS24220	34461..35432	-	972	WP_163516419.1	plasmid stabilization protein	-
HV284_RS24225	35597..35893	+	297	WP_181475199.1	hypothetical protein	-
HV284_RS24230	35969..36652	+	684	WP_149505782.1	DNA methylase	-
HV284_RS24235	36653..36874	+	222	WP_001558391.1	hypothetical protein	-
HV284_RS24240	36888..37322	+	435	WP_105279872.1	DUF1380 family protein	-
HV284_RS24245	37849..38151	+	303	WP_001666994.1	antirestriction protein	-
HV284_RS24250	38200..38391	+	192	WP_021548775.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ / papC / papH / papH / papC	1..116663	116663

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11087.07 Da
• Isoelectric Point: 10.4701

>T162842 WP_134901167.1 NZ_CP056160:33157-33444 [Escherichia marmotae]
MTYTVKFRDDALKEWLKLDKAIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T162842 NZ_CP072138:526160-526579 [Agrobacterium sp. S7/73]
ATGATCCTGCTTGACACCAACGTCACATCCGAACCTTGGAAACCGGTTCCGGACGAGGCGGTGATTGCCTGGTTGGACGC
CCAAGCTGTCGAAACGCTGTTCATCTCCGCAATAACGATTGCGGAACTTCGCTTCGAAATTGCTGCTATGCCTTCAGGCA
GACGGCAAACTATCCTCCGCGACCGTATCGAGGGCGAGGTGCTGCCACACTTTTCCGGCCGCATCCTGTCCTTCGATCTG
GCTACTTCTCAGTTCTACTCTGAACTGATGGCACGCGCTCGAGCGTCTGGGAAAGCGATCGGCACGGCCGATGGCTACAT
TGCAGCCACAGCCGTAGCAAATGGCCTCACGATCTCAACTCGCGATACAAGCCCTTTCGAGGCTGCCGGCGTGAAAGTGA
TCAACCCCTGGTCCAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT162842 WP_001132900.1 NZ_CP056160:32909-33160 [Escherichia marmotae]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT162842 NZ_CP072138:525909-526163 [Agrobacterium sp. S7/73]
ATGCCGGCAGTCACCATTCGTAACCTTTCTGAAGAAACACATCGCGCTCTGAGGGTGCGAGCGGCTCATCATGGCCGGAG
CACGGAGGCCGAGATTCGTGACATTATTGAAGCGGCTGTTCGTCCTCCGGAACGTATTCAGCTCGGGTCCTTGCTAGCGG
CCATTGGTCGCGACGCAGAGCTTTCCAACAGCGACGTTGAAGCTCTTCAGAAAAGTCGCGACAAGACACCTGCTAAGCCG
ATGACCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: V0VIP2