Detailed information of TA system TA162839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3910682..3911460
• Replicon: chromosome
• Accession: NZ_CP056159
• Organism: Escherichia marmotae strain RHBSTW-00814

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV284_RS18950
• Protein ID: WP_001517064.1
• Coordinates: 3910972..3911460 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: HV284_RS18945
• Protein ID: WP_001110459.1
• Coordinates: 3910682..3910975 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV284_RS18925	3906443..3907426	-	984	WP_001325875.1	KpsF/GutQ family sugar-phosphate isomerase	-
HV284_RS18930	3908251..3908586	-	336	WP_000789899.1	hypothetical protein	-
HV284_RS18935	3908583..3909056	-	474	WP_000143249.1	hypothetical protein	-
HV284_RS18940	3909650..3910147	+	498	WP_181475134.1	type VI secretion system contractile sheath small subunit	-
HV284_RS18945	3910682..3910975	+	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
HV284_RS18950	3910972..3911460	+	489	WP_001517064.1	GNAT family N-acetyltransferase	Toxin
HV284_RS18955	3912041..3912331	-	291	WP_157941656.1	hypothetical protein	-
HV284_RS18960	3912355..3913890	-	1536	WP_097463307.1	EAL domain-containing protein	-
HV284_RS18965	3913961..3914164	-	204	Protein_3703	DUF4942 domain-containing protein	-
HV284_RS18970	3914259..3915758	+	1500	WP_181474344.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17521.41 Da
• Isoelectric Point: 7.7535

>T162839 WP_001517064.1 NZ_CP056159:3910972-3911460 [Escherichia marmotae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T162839 NZ_CP072138:c145221-144739 [Agrobacterium sp. S7/73]
ATGCTTTCCGCTCCGGCGCTTCTTGAGGAGGCGCATGACCTTGACCTGTTCCAGAGCGGAAACGATACGCTCGATGAATG
GCTGCGCCGCCGCGCGCGACCCAATCAGGTCAGCGGTGCGTCCCGGACCTATGTCGTCGCCGAGGGGCAGCGCGTCGTCG
GCTATTATTGCCTTGCGTCTGGCGCGCTCGATCTGGCCGATGCGCCGGGATCGGTTCGGCGAAACATGCCAGATCCAATT
CCCATGGCGGTTCTTGGTCGGCTTGCCATCGATCGCGGTTGGCAGGGCAGGGGATTGGGCGCGGCGCTTTTGCAGGATGC
CGTCCTTCGCACATCGCAGGCGGCGTCCATCATGGGCATTCGCGGCCTTCTTGTGCATGCGATTTCCGAAGACGCCAAAG
CCTTTTACGAGCACTATGGTTTCCTATGCTCTCCCAATCAACCCATGACGCTGGTGCTCTCGCTTAAGATCAGGACCGGA
TAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT162839 WP_001110459.1 NZ_CP056159:3910682-3910975 [Escherichia marmotae]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT162839 NZ_CP072138:c145505-145221 [Agrobacterium sp. S7/73]
ATGGCCACAAAATCGTTGACGGACTCACATGCCAAGCCGGTCCACCTGCGTATCCGCGAGGACGTGCGCAAGGTGATCGA
TCGCGCCGCCAGCATTCGCGGCAAGAGCCGCACGGATTTCATGATCGATGCGGCTTACCGCGCTGCGGAAGACGCCTTGC
TCGACCAAACTCTTGTGCGGGTTGATGAGGACAGCTATCGCCACTTCCTCTCCGTCCTCGATCAGCCGCCGAATGGGGAA
GGTTTCGAGCGGTTGATGAACGCGCCCAAGCCTTGGCAGTCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5