Detailed information of TA system TA162837

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2662876..2663711
• Replicon: chromosome
• Accession: NZ_CP056159
• Organism: Escherichia marmotae strain RHBSTW-00814

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV284_RS13095
• Protein ID: WP_170898765.1
• Coordinates: 2663334..2663711 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7H9K7L9
• Locus tag: HV284_RS13090
• Protein ID: WP_059268623.1
• Coordinates: 2662876..2663244 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV284_RS13050	2658007..2658891	+	885	WP_181474154.1	50S ribosome-binding GTPase	-
HV284_RS13055	2659010..2659687	+	678	WP_001601175.1	hypothetical protein	-
HV284_RS13060	2659693..2659926	+	234	WP_181474155.1	DUF905 family protein	-
HV284_RS13065	2660026..2660844	+	819	WP_181474156.1	DUF945 domain-containing protein	-
HV284_RS13070	2660935..2661420	+	486	WP_053272402.1	antirestriction protein	-
HV284_RS13075	2661435..2661911	+	477	WP_181474157.1	RadC family protein	-
HV284_RS13080	2661980..2662201	+	222	WP_001601167.1	DUF987 domain-containing protein	-
HV284_RS13085	2662216..2662860	+	645	WP_170898767.1	antitoxin of toxin-antitoxin stability system	-
HV284_RS13090	2662876..2663244	+	369	WP_059268623.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV284_RS13095	2663334..2663711	+	378	WP_170898765.1	TA system toxin CbtA family protein	Toxin
HV284_RS13100	2663708..2664196	+	489	WP_170898763.1	hypothetical protein	-
HV284_RS13105	2664208..2664375	+	168	WP_181474158.1	hypothetical protein	-
HV284_RS13110	2664360..2665280	+	921	Protein_2573	EAL domain-containing protein	-
HV284_RS13115	2666069..2667607	+	1539	WP_061090610.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	papD / papC / papH / clpV/tssH / hcp/tssD / vipB/tssC / vipA/tssB	2504142..2665461	161319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14162.00 Da
• Isoelectric Point: 6.6312

>T162837 WP_170898765.1 NZ_CP056159:2663334-2663711 [Escherichia marmotae]
MNTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T162837 NZ_CP072130:c5442036-5441653 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13812.64 Da
• Isoelectric Point: 5.8808

>AT162837 WP_059268623.1 NZ_CP056159:2662876-2663244 [Escherichia marmotae]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT162837 NZ_CP072130:c5442278-5442036 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7H9K7L9