Detailed information of TA system TA162836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2310776..2311352
• Replicon: chromosome
• Accession: NZ_CP056159
• Organism: Escherichia marmotae strain RHBSTW-00814

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV284_RS11350
• Protein ID: WP_105270768.1
• Coordinates: 2311065..2311352 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: HV284_RS11345
• Protein ID: WP_000063150.1
• Coordinates: 2310776..2311078 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV284_RS11330	2307290..2309440	+	2151	WP_019841686.1	pyruvate/proton symporter BtsT	-
HV284_RS11335	2309572..2309775	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HV284_RS11340	2309786..2310742	+	957	WP_001297640.1	GTPase	-
HV284_RS11345	2310776..2311078	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
HV284_RS11350	2311065..2311352	-	288	WP_105270768.1	BrnT family toxin	Toxin
HV284_RS11355	2311549..2312715	+	1167	WP_181475054.1	restriction endonuclease	-
HV284_RS11360	2312779..2314398	+	1620	WP_181475055.1	type I restriction-modification system subunit M	-
HV284_RS11365	2314388..2315749	+	1362	WP_000535394.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11110.64 Da
• Isoelectric Point: 8.6424

>T162836 WP_105270768.1 NZ_CP056159:c2311352-2311065 [Escherichia marmotae]
MPMEFEWDANKVKSNRVKHGIRFEDAVLVFDDPQHLSQQDRVGNGEYRWQTIGLVHGIVVILVAHIIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T162836 NZ_CP072130:c5117682-5116765 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCTCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCTAATCTGCAACAACAG
GCAGGTATGAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATGTCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT162836 WP_000063150.1 NZ_CP056159:c2311078-2310776 [Escherichia marmotae]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT162836 NZ_CP072130:c5118446-5117688 [Klebsiella variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAATGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4