Detailed information of TA system TA162829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 682043..682681
• Replicon: chromosome
• Accession: NZ_CP056159
• Organism: Escherichia marmotae strain RHBSTW-00814

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: HV284_RS03465
• Protein ID: WP_000813795.1
• Coordinates: 682505..682681 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV284_RS03460
• Protein ID: WP_077627724.1
• Coordinates: 682043..682459 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV284_RS03440	677198..678139	-	942	WP_181474737.1	ABC transporter permease	-
HV284_RS03445	678140..679153	-	1014	WP_000220424.1	ABC transporter ATP-binding protein	-
HV284_RS03450	679171..680316	-	1146	WP_000047463.1	ABC transporter substrate-binding protein	-
HV284_RS03455	680555..681964	-	1410	WP_061091001.1	PLP-dependent aminotransferase family protein	-
HV284_RS03460	682043..682459	-	417	WP_077627724.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HV284_RS03465	682505..682681	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HV284_RS03470	682903..683133	+	231	WP_181474738.1	DUF2554 family protein	-
HV284_RS03475	683227..685188	-	1962	WP_181475093.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HV284_RS03480	685261..685797	-	537	WP_000429073.1	XRE family transcriptional regulator	-
HV284_RS03485	685850..687061	+	1212	WP_105266767.1	benzoate/H(+) symporter BenE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T162829 WP_000813795.1 NZ_CP056159:c682681-682505 [Escherichia marmotae]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T162829 NZ_CP072130:354687-355034 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15250.62 Da
• Isoelectric Point: 4.5908

>AT162829 WP_077627724.1 NZ_CP056159:c682459-682043 [Escherichia marmotae]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNNNDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT162829 NZ_CP072130:355034-355333 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1