Detailed information of TA system TA162815

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 66816..67525
• Replicon: plasmid pRHBSTW-00844_2
• Accession: NZ_CP056156
• Organism: Enterobacter hormaechei strain RHBSTW-00844

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7D6YAQ1
• Locus tag: HV295_RS21475
• Protein ID: WP_071886833.1
• Coordinates: 67157..67525 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV295_RS21470
• Protein ID: WP_047051179.1
• Coordinates: 66816..67136 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV295_RS21430	62376..63210	+	835	Protein_54	DUF945 domain-containing protein	-
HV295_RS21435	63412..64116	+	705	WP_047051185.1	WYL domain-containing protein	-
HV295_RS21440	64113..64727	+	615	WP_047051184.1	hypothetical protein	-
HV295_RS21445	64816..65226	+	411	WP_047051183.1	hypothetical protein	-
HV295_RS21450	65303..65539	+	237	WP_047051182.1	DUF905 domain-containing protein	-
HV295_RS21455	65619..66077	+	459	WP_047051181.1	antirestriction protein	-
HV295_RS21460	66086..66568	+	483	WP_075211782.1	DNA repair protein RadC	-
HV295_RS21465	66577..66798	+	222	WP_016243789.1	DUF987 domain-containing protein	-
HV295_RS21470	66816..67136	+	321	WP_047051179.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV295_RS21475	67157..67525	+	369	WP_071886833.1	TA system toxin CbtA family protein	Toxin
HV295_RS21480	67871..68617	-	747	WP_047051178.1	class I SAM-dependent methyltransferase	-
HV295_RS21485	68628..68885	-	258	WP_003863178.1	YjhX family toxin	-
HV295_RS21490	69145..71082	-	1938	WP_181522303.1	bifunctional diguanylate cyclase/phosphodiesterase	-
HV295_RS21495	71100..71594	-	495	WP_047051176.1	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	IlpA / gmhA/lpcA / acrB / acrA / fimC / fimD / fimH / gtrB / gtrA / lpxD / lpxA	1..361638	361638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (6-105)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13738.90 Da
• Isoelectric Point: 8.1347

>T162815 WP_071886833.1 NZ_CP056156:67157-67525 [Enterobacter hormaechei]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDQTVIQEHIDAGITLANAVNFLVEKYELVRIDRTGF
TSQEQAPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSRQ

Nucleotide

• Length: 369 bp

>T162815 NZ_CP072119:2282762-2283094 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11901.60 Da
• Isoelectric Point: 7.2633

>AT162815 WP_047051179.1 NZ_CP056156:66816-67136 [Enterobacter hormaechei]
MRSNATWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFISLMESMLTTGEMNPRHAHCVTLY
RNGFTCEADTLGSCGYLYIVVYPTQS

Nucleotide

• Length: 321 bp

>AT162815 NZ_CP072119:2282504-2282761 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Structures

Toxin

• AlphaFold DB: A0A7D6YAQ1