Detailed information of TA system TA162792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 96314..96839
• Replicon: plasmid pRHBSTW-00853_2
• Accession: NZ_CP056151
• Organism: Klebsiella grimontii strain RHBSTW-00853

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: HV299_RS29610
• Protein ID: WP_004197633.1
• Coordinates: 96314..96619 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV299_RS29615
• Protein ID: WP_004197642.1
• Coordinates: 96621..96839 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV299_RS29575	91505..92848	-	1344	WP_077259867.1	ISNCY family transposase	-
HV299_RS29580	93055..93537	-	483	WP_049083183.1	GNAT family N-acetyltransferase	-
HV299_RS29585	93525..93791	-	267	WP_049083184.1	DUF1778 domain-containing protein	-
HV299_RS29590	93878..94657	+	780	WP_176392677.1	hypothetical protein	-
HV299_RS29595	94651..95348	-	698	Protein_109	IS1 family transposase	-
HV299_RS29600	95403..95717	-	315	WP_074181342.1	hypothetical protein	-
HV299_RS29605	95744..96145	-	402	WP_177343058.1	hypothetical protein	-
HV299_RS29610	96314..96619	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV299_RS29615	96621..96839	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV299_RS29625	98502..99035	-	534	WP_004118098.1	carboxymuconolactone decarboxylase family protein	-
HV299_RS29630	99156..100064	+	909	WP_004118099.1	AraC family transcriptional regulator	-
HV299_RS29635	100083..101018	-	936	WP_004118100.1	GlxA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..201629	201629
	-	inside	IScluster/Tn	-	-	88118..98284	10166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T162792 WP_004197633.1 NZ_CP056151:c96619-96314 [Klebsiella grimontii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T162792 NZ_CP072113:c174037-173624 [Yimella sp. cx-51]
GTGAACGTGGTGCTGGCAATGCACCGCCAGGACCACCCGCAGCATTCGCCGGTTCGCGCATGGTTCGACCAGGTCGTCGA
AAATGGCGAAGATTTTGGCGTGCCGTTGACTGTGTGGGGCTCGTTCCTAAGAATCACGACCAACCGGCGAGTATTCAAAG
TGCCAACCCCATTGCATGACGCCTTCACCTTTATCGACGCGCTCCGCGGCCAACCTGGGCACATCCAACTCGAGCCCGGC
ATTCGTCACCTTGTGCTGCTTCAGCGCATCGCCGAACGTTCAGACGCCTATGGTGACCTCGTTCCAGACGTCGTGCTGGC
AGCCATCGCCGCGGAGGCTGGGGCAAAGGTGGCGTCGCTCGATCGCGACTTCGCTCGTATCGCAGACATTGAGGTCGTTC
GACCAGTGCAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT162792 WP_004197642.1 NZ_CP056151:c96839-96621 [Klebsiella grimontii]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT162792 NZ_CP072113:c174308-174054 [Yimella sp. cx-51]
ATGCGCACCACGCTCTCCATCGACGACGAACTCCTCGCGCGGGCCCGCGAACGCGCCCGCGAGCGGGGCATCACGTTGGG
TGCAGTTGTGGAGAATGCCCTCCGCCGCGACTTTGCGAGCGAGGTCCGGACCGACGCTCCTCCCCTTCCAGTCTTCACAA
AGGGGAGCGGGCCCAAACCTGGAATTGATCTGACTTCTAATCGCGTCCTCCACGAGGTGCTCGACGAGGATCTGCCCCTC
GATCGGCTGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5