Detailed information of TA system TA162791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 93055..93791
• Replicon: plasmid pRHBSTW-00853_2
• Accession: NZ_CP056151
• Organism: Klebsiella grimontii strain RHBSTW-00853

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV299_RS29580
• Protein ID: WP_049083183.1
• Coordinates: 93055..93537 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HV299_RS29585
• Protein ID: WP_049083184.1
• Coordinates: 93525..93791 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV299_RS29555	88118..89086	+	969	WP_177939556.1	IS5 family transposase	-
HV299_RS29560	89337..89747	+	411	WP_040210104.1	hypothetical protein	-
HV299_RS29565	90035..90607	+	573	WP_032676095.1	recombinase family protein	-
HV299_RS29570	90616..91434	+	819	WP_023307220.1	abortive infection family protein	-
HV299_RS29575	91505..92848	-	1344	WP_077259867.1	ISNCY family transposase	-
HV299_RS29580	93055..93537	-	483	WP_049083183.1	GNAT family N-acetyltransferase	Toxin
HV299_RS29585	93525..93791	-	267	WP_049083184.1	DUF1778 domain-containing protein	Antitoxin
HV299_RS29590	93878..94657	+	780	WP_176392677.1	hypothetical protein	-
HV299_RS29595	94651..95348	-	698	Protein_109	IS1 family transposase	-
HV299_RS29600	95403..95717	-	315	WP_074181342.1	hypothetical protein	-
HV299_RS29605	95744..96145	-	402	WP_177343058.1	hypothetical protein	-
HV299_RS29610	96314..96619	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
HV299_RS29615	96621..96839	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..201629	201629
	-	inside	IScluster/Tn	-	-	88118..98284	10166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17326.90 Da
• Isoelectric Point: 9.1188

>T162791 WP_049083183.1 NZ_CP056151:c93537-93055 [Klebsiella grimontii]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T162791 NZ_CP072111:937492-937746 [Corynebacterium hindlerae]
ATGCTCCTCACTTGGGATGAGAATGCCTGGGAAGACTATCTGTGGTGGCAAGCGCACGACCGGAAAGTCTTGAAGCGGAT
CAATGCTCTAATCAAGGACATTGCCCGGAATGGCAACGAGGGAATAGGTAAGCCCGAGCCATTGAAACATGAATTTGCCG
GATTCTGGTCTCGGCGAATCACTGAAGAGCATCGCCTCGTCTACCGAGTGGTTGAAAACGACATCACTGTGCTGGCTTGT
CGTTATCACTATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10117.61 Da
• Isoelectric Point: 4.6437

>AT162791 WP_049083184.1 NZ_CP056151:c93791-93525 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT162791 NZ_CP072111:937232-937486 [Corynebacterium hindlerae]
ATGAAGACGATCAGTTACACCGAGTCGCGTGCGAATTATGCGCGGGTCTTGGACGACGTTGTCAATGATCGTGAGCCTGT
CACCATTACGCGAGCCGGGCACGAATCAGTCGTGATGATGTCCCTAGATGATTATTCGTCATTGCAGGAAACTGCTTATC
TGATGCGGTCACCGGCAAATGCTCGACGGCTTTTCGACGCGCTGGAGCGTGTGAAAGCTGGAGACGTAACAAAGCATGAG
CTCATCGAGGACTAA