Detailed information of TA system TA162790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 33187..33709
• Replicon: plasmid pRHBSTW-00853_2
• Accession: NZ_CP056151
• Organism: Klebsiella grimontii strain RHBSTW-00853

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HV299_RS29260
• Protein ID: WP_004187019.1
• Coordinates: 33187..33471 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV299_RS29265
• Protein ID: WP_004187025.1
• Coordinates: 33461..33709 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV299_RS29235	28262..29185	-	924	WP_023328909.1	cation diffusion facilitator family transporter	-
HV299_RS29240	29446..29766	+	321	WP_049084165.1	metalloregulator ArsR/SmtB family transcription factor	-
HV299_RS29245	29812..31101	+	1290	WP_035897525.1	arsenite efflux transporter membrane subunit ArsB	-
HV299_RS29250	31114..31542	+	429	WP_035897523.1	glutaredoxin-dependent arsenate reductase	-
HV299_RS29255	31828..33171	+	1344	WP_064360326.1	ISNCY family transposase	-
HV299_RS29260	33187..33471	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV299_RS29265	33461..33709	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV299_RS29270	34087..34485	-	399	WP_049084174.1	helix-turn-helix domain-containing protein	-
HV299_RS29275	34534..35880	-	1347	WP_077259864.1	ISNCY family transposase	-
HV299_RS29280	36336..37535	-	1200	WP_053086565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..201629	201629

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T162790 WP_004187019.1 NZ_CP056151:c33471-33187 [Klebsiella grimontii]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T162790 NZ_CP072111:937232-937486 [Corynebacterium hindlerae]
ATGAAGACGATCAGTTACACCGAGTCGCGTGCGAATTATGCGCGGGTCTTGGACGACGTTGTCAATGATCGTGAGCCTGT
CACCATTACGCGAGCCGGGCACGAATCAGTCGTGATGATGTCCCTAGATGATTATTCGTCATTGCAGGAAACTGCTTATC
TGATGCGGTCACCGGCAAATGCTCGACGGCTTTTCGACGCGCTGGAGCGTGTGAAAGCTGGAGACGTAACAAAGCATGAG
CTCATCGAGGACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT162790 WP_004187025.1 NZ_CP056151:c33709-33461 [Klebsiella grimontii]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT162790 NZ_CP072111:937492-937746 [Corynebacterium hindlerae]
ATGCTCCTCACTTGGGATGAGAATGCCTGGGAAGACTATCTGTGGTGGCAAGCGCACGACCGGAAAGTCTTGAAGCGGAT
CAATGCTCTAATCAAGGACATTGCCCGGAATGGCAACGAGGGAATAGGTAAGCCCGAGCCATTGAAACATGAATTTGCCG
GATTCTGGTCTCGGCGAATCACTGAAGAGCATCGCCTCGTCTACCGAGTGGTTGAAAACGACATCACTGTGCTGGCTTGT
CGTTATCACTATTAG

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4