Detailed information of TA system TA162789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 22907..23817
• Replicon: plasmid pRHBSTW-00853_2
• Accession: NZ_CP056151
• Organism: Klebsiella grimontii strain RHBSTW-00853

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MBW7
• Locus tag: HV299_RS29205
• Protein ID: WP_023329005.1
• Coordinates: 23347..23817 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A9J6S5L1
• Locus tag: HV299_RS29200
• Protein ID: WP_049084156.1
• Coordinates: 22907..23350 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV299_RS29175	18721..19059	-	339	WP_032738440.1	hypothetical protein	-
HV299_RS29180	19258..19979	+	722	Protein_26	DUF969 domain-containing protein	-
HV299_RS29185	19976..20932	+	957	Protein_27	DUF979 domain-containing protein	-
HV299_RS29190	20942..21937	+	996	WP_049084154.1	DUF2891 domain-containing protein	-
HV299_RS29195	22098..22785	+	688	Protein_29	DUF4113 domain-containing protein	-
HV299_RS29200	22907..23350	+	444	WP_049084156.1	DUF2384 domain-containing protein	Antitoxin
HV299_RS29205	23347..23817	+	471	WP_023329005.1	RES family NAD+ phosphorylase	Toxin
HV299_RS29210	23928..24187	-	260	Protein_32	hypothetical protein	-
HV299_RS29215	24882..26257	+	1376	WP_154957233.1	IS3 family transposase	-
HV299_RS29220	26506..27081	-	576	WP_032731970.1	cytochrome b/b6 domain-containing protein	-
HV299_RS29225	27229..27534	+	306	WP_023328906.1	metalloregulator ArsR/SmtB family transcription factor	-
HV299_RS29230	27536..28069	+	534	WP_049100197.1	DUF2892 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..201629	201629
	-	flank	IS/Tn	-	-	25499..26257	758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17459.70 Da
• Isoelectric Point: 4.4829

>T162789 WP_023329005.1 NZ_CP056151:23347-23817 [Klebsiella grimontii]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T162789 NZ_CP072110:3310947-3311234 [Psychrosphaera ytuae]
ATGACTTATAAGTTAAAGTTTCTTCCATTAGCTAAAAAAGAATGGGATAAATTAGCTGAGCCACTTAAAAAGCAATTTAA
AAATAAACTTGCTGAAAGATTAGAAAACCCTCATGTACCGTCAGACAAACTAAGAGGATATGAATCAGTCTATAAGATCA
AACTACGTACAGCAGGTTATCGATTAGCTTATGAAGTCCTCGATAATGAAGTCGTAGTATACGTATTAGCTGTAGGAAAG
CGTGATAAAAATGCTATTTATAAAAAGTTAGCATCTCGCATGGAGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16447.80 Da
• Isoelectric Point: 10.2498

>AT162789 WP_049084156.1 NZ_CP056151:22907-23350 [Klebsiella grimontii]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT162789 NZ_CP072110:3310706-3310957 [Psychrosphaera ytuae]
ATGAGGCAAGTTTTAGCTGACTGTTCAGCAAGTATTTCTGAACTAAAAAAGAACCCTACTGCTTTACTGAATGAGGCGGA
TGGAGCTGCAATTGCAATATTAAATCACAATAAACCTGCTGCATATCTGGTTCCTGCAGAAACTTATGAGTGGTTAATGA
GTGTTGTCGATGATTACGAGTTAGCAAAAGTGGTAGAAAAGCGTCGCTCTGATCTATCAAACGCAATTGAAGTTAACTTA
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBW7