Detailed information of TA system TA162786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4930401..4931044
• Replicon: chromosome
• Accession: NZ_CP056150
• Organism: Klebsiella grimontii strain RHBSTW-00853

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV299_RS23860
• Protein ID: WP_154916276.1
• Coordinates: 4930401..4930817 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HV299_RS23865
• Protein ID: WP_098067465.1
• Coordinates: 4930814..4931044 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV299_RS23845	4926634..4928031	+	1398	WP_004132593.1	MFS transporter	-
HV299_RS23850	4928155..4929495	+	1341	WP_154916277.1	carbohydrate porin	-
HV299_RS23855	4929633..4929848	-	216	Protein_4697	LysR family transcriptional regulator	-
HV299_RS23860	4930401..4930817	-	417	WP_154916276.1	PIN domain-containing protein	Toxin
HV299_RS23865	4930814..4931044	-	231	WP_098067465.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV299_RS23870	4931298..4931936	+	639	WP_154916275.1	hypothetical protein	-
HV299_RS23875	4932479..4933771	-	1293	WP_154956990.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15088.63 Da
• Isoelectric Point: 7.2457

>T162786 WP_154916276.1 NZ_CP056150:c4930817-4930401 [Klebsiella grimontii]
MKKTWMLDTNICSFIMREQPESVLKRLEQAMLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDALCARLDAVLPLEHS
AVDATTDIKVALRLVGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLILEDWVK

Nucleotide

• Length: 417 bp

>T162786 NZ_CP072065:243936-244286 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8669.73 Da
• Isoelectric Point: 4.6412

>AT162786 WP_098067465.1 NZ_CP056150:c4931044-4930814 [Klebsiella grimontii]
MHTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGNSIILRPVRPTWSSLASLEKADADFLKEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT162786 NZ_CP072065:243644-243931 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA