Detailed information of TA system TA162771

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4125035..4125714
• Replicon: chromosome
• Accession: NZ_CP056148
• Organism: Enterobacter roggenkampii strain RHBSTW-00872

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV308_RS19955
• Protein ID: WP_047743670.1
• Coordinates: 4125035..4125376 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV308_RS19960
• Protein ID: WP_048981704.1
• Coordinates: 4125397..4125714 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV308_RS19935	4120323..4122068	+	1746	WP_023294585.1	DNA primase	-
HV308_RS19940	4122223..4124070	+	1848	WP_008503184.1	RNA polymerase sigma factor RpoD	-
HV308_RS19945	4124167..4124673	-	507	WP_008503183.1	G/U mismatch-specific DNA glycosylase	-
HV308_RS19955	4125035..4125376	-	342	WP_047743670.1	TA system toxin CbtA family protein	Toxin
HV308_RS19960	4125397..4125714	-	318	WP_048981704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV308_RS19965	4125733..4125954	-	222	WP_048981705.1	DUF987 domain-containing protein	-
HV308_RS19970	4125963..4126439	-	477	WP_048981706.1	RadC family protein	-
HV308_RS19975	4126455..4126913	-	459	WP_048981707.1	antirestriction protein	-
HV308_RS19980	4127016..4127255	-	240	WP_047743666.1	DUF905 domain-containing protein	-
HV308_RS19985	4127332..4127799	-	468	WP_047743665.1	hypothetical protein	-
HV308_RS19990	4127823..4128263	-	441	WP_048981708.1	hypothetical protein	-
HV308_RS19995	4128263..4128499	-	237	WP_047743663.1	hypothetical protein	-
HV308_RS20000	4128540..4129241	-	702	WP_048981709.1	WYL domain-containing protein	-
HV308_RS20005	4129458..4130279	-	822	WP_048981710.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD / bcfA	4125035..4175093	50058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13110.17 Da
• Isoelectric Point: 10.3379

>T162771 WP_047743670.1 NZ_CP056148:c4125376-4125035 [Enterobacter roggenkampii]
MKTLPATTQRAVRRCLSPVAVWQMLLTRLLEQHYGLTLNDTLFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T162771 NZ_CP072054:3741797-3742204 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11987.55 Da
• Isoelectric Point: 6.9465

>AT162771 WP_048981704.1 NZ_CP056148:c4125714-4125397 [Enterobacter roggenkampii]
MSNTIWGLQREFSPRLGARLVQEGNRLHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT162771 NZ_CP072054:3741550-3741816 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA