Detailed information of TA system TA162760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1525930..1526843
• Replicon: chromosome
• Accession: NZ_CP056148
• Organism: Enterobacter roggenkampii strain RHBSTW-00872

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV308_RS07335
• Protein ID: WP_059361206.1
• Coordinates: 1525930..1526403 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8B2SER9
• Locus tag: HV308_RS07340
• Protein ID: WP_023327017.1
• Coordinates: 1526400..1526843 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV308_RS07315	1522090..1523400	+	1311	WP_059361211.1	type I-F CRISPR-associated protein Csy1	-
HV308_RS07320	1523397..1524332	+	936	WP_122008404.1	type I-F CRISPR-associated protein Csy2	-
HV308_RS07325	1524346..1525344	+	999	WP_181548435.1	type I-F CRISPR-associated protein Csy3	-
HV308_RS07330	1525354..1525908	+	555	WP_059361208.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
HV308_RS07335	1525930..1526403	-	474	WP_059361206.1	RES domain-containing protein	Toxin
HV308_RS07340	1526400..1526843	-	444	WP_023327017.1	DUF2384 domain-containing protein	Antitoxin
HV308_RS07345	1528244..1528462	-	219	WP_002211347.1	translation initiation factor IF-1	-
HV308_RS07350	1528747..1529451	-	705	WP_008499991.1	leucyl/phenylalanyl-tRNA--protein transferase	-
HV308_RS07355	1529497..1531218	-	1722	WP_122008403.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17467.78 Da
• Isoelectric Point: 5.4786

>T162760 WP_059361206.1 NZ_CP056148:c1526403-1525930 [Enterobacter roggenkampii]
MIFYRLVTGRYASGAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSIDIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQANSALALILPSCIIPYENNAILNPLHPAFHSALNSVQQLPFIFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T162760 NZ_CP072054:1397815-1397991 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16352.68 Da
• Isoelectric Point: 7.2062

>AT162760 WP_023327017.1 NZ_CP056148:c1526843-1526400 [Enterobacter roggenkampii]
MRTWIPAQKPADNALWRYAGLPANRGMRLIEFLNQGLPVSVLDNIHEWTSMSKADILRVTGINERNVARRKSAGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWTWLQAPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT162760 NZ_CP072054:1398037-1398453 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2SER9