Detailed information of TA system TA162757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 45740..46401
• Replicon: chromosome
• Accession: NZ_CP056148
• Organism: Enterobacter roggenkampii strain RHBSTW-00872

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV308_RS00235
• Protein ID: WP_155020498.1
• Coordinates: 46003..46401 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A156KC23
• Locus tag: HV308_RS00230
• Protein ID: WP_032675702.1
• Coordinates: 45740..46006 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV308_RS00205	41793..43121	-	1329	WP_025910681.1	PTS sugar transporter subunit IIC	-
HV308_RS00210	43133..43447	-	315	WP_155020497.1	PTS sugar transporter subunit IIB	-
HV308_RS00215	43741..44691	+	951	WP_023293714.1	LacI family transcriptional regulator	-
HV308_RS00220	44753..45049	-	297	WP_059365122.1	hypothetical protein	-
HV308_RS00225	45191..45610	+	420	WP_008500129.1	GNAT family N-acetyltransferase	-
HV308_RS00230	45740..46006	+	267	WP_032675702.1	virulence protein	Antitoxin
HV308_RS00235	46003..46401	+	399	WP_155020498.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV308_RS00240	46541..47341	+	801	WP_155020499.1	lipoprotein NlpA	-
HV308_RS00245	47464..48243	+	780	WP_126348546.1	MBL fold metallo-hydrolase	-
HV308_RS00250	48270..49223	+	954	WP_044597401.1	helix-turn-helix domain-containing protein	-
HV308_RS00255	49372..50388	+	1017	WP_123244595.1	IS5 family transposase	-
HV308_RS00260	50536..50652	-	117	Protein_51	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	49408..50388	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14826.07 Da
• Isoelectric Point: 6.8547

>T162757 WP_155020498.1 NZ_CP056148:46003-46401 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTIKICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T162757 NZ_CP072054:c1156978-1156871 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.34 Da
• Isoelectric Point: 4.6310

>AT162757 WP_032675702.1 NZ_CP056148:45740-46006 [Enterobacter roggenkampii]
MERTAKLFRKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT162757 NZ_CP072054:1157025-1157091 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A156KC23