Detailed information of TA system TA162755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5429652..5430277
• Replicon: chromosome
• Accession: NZ_CP056145
• Organism: Klebsiella variicola strain RHBSTW-00883

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV312_RS26105
• Protein ID: WP_023323421.1
• Coordinates: 5429652..5430035 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: HV312_RS26110
• Protein ID: WP_004150355.1
• Coordinates: 5430035..5430277 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV312_RS26090	5427018..5427920	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HV312_RS26095	5427917..5428552	+	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
HV312_RS26100	5428549..5429478	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HV312_RS26105	5429652..5430035	-	384	WP_023323421.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV312_RS26110	5430035..5430277	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV312_RS26115	5430482..5431399	+	918	WP_032733990.1	alpha/beta hydrolase	-
HV312_RS26120	5431414..5432355	-	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
HV312_RS26125	5432400..5432837	-	438	WP_012543288.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV312_RS26130	5432834..5433694	-	861	WP_008807907.1	virulence factor BrkB family protein	-
HV312_RS26135	5433688..5434287	-	600	WP_064176466.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14403.65 Da
• Isoelectric Point: 7.2018

>T162755 WP_023323421.1 NZ_CP056145:c5430035-5429652 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLYRLELITRNTKDFAGIPGVVTPYELHPE

Nucleotide

• Length: 384 bp

>T162755 NZ_CP072054:c382291-382073 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT162755 WP_004150355.1 NZ_CP056145:c5430277-5430035 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT162755 NZ_CP072054:c382691-382317 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9