Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324556..325142 Replicon chromosome
Accession NZ_CP056145
Organism Klebsiella variicola strain RHBSTW-00883

Toxin (Protein)


Gene name doc Uniprot ID A0A2W7TCK5
Locus tag HV312_RS01485 Protein ID WP_008806973.1
Coordinates 324774..325142 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HV312_RS01480 Protein ID WP_004174006.1
Coordinates 324556..324777 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV312_RS01460 320713..321639 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HV312_RS01465 321636..322913 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
HV312_RS01470 322910..323677 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HV312_RS01475 323679..324392 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HV312_RS01480 324556..324777 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HV312_RS01485 324774..325142 + 369 WP_008806973.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HV312_RS01490 325434..326750 + 1317 WP_048268284.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HV312_RS01495 326857..327744 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HV312_RS01500 327741..328586 + 846 WP_048268283.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HV312_RS01505 328588..329658 + 1071 WP_044649551.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 319542..330395 10853


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13639.01 Da        Isoelectric Point: 7.3191

>T162740 WP_008806973.1 NZ_CP056145:324774-325142 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T162740 NZ_CP071992:2033728-2033916 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAGAGAAGAACGAAAAGAATCTTATCTAGGTTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT162740 WP_004174006.1 NZ_CP056145:324556-324777 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT162740 NZ_CP071992:2033446-2033703 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATGTATTCAACTTGATATTCACCATTTTATTTTTTACATTTAACATTCTCATAACCTATAA
CGCAAATATTGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGCTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTATATTATATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W7TCK5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References