Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 280999..281630 Replicon chromosome
Accession NZ_CP056145
Organism Klebsiella variicola strain RHBSTW-00883

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag HV312_RS01265 Protein ID WP_044650325.1
Coordinates 280999..281274 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag HV312_RS01270 Protein ID WP_004181489.1
Coordinates 281271..281630 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV312_RS01245 276235..276570 + 336 WP_002921203.1 universal stress protein UspB -
HV312_RS01250 276630..278126 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
HV312_RS01255 278356..279549 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
HV312_RS01260 279589..280602 - 1014 WP_012540371.1 magnesium transporter -
HV312_RS01265 280999..281274 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
HV312_RS01270 281271..281630 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HV312_RS01275 281658..282059 - 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
HV312_RS01280 282047..282838 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
HV312_RS01285 282835..283599 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
HV312_RS01290 283599..284432 - 834 WP_088717475.1 nickel ABC transporter permease subunit NikC -
HV312_RS01295 284429..285373 - 945 WP_032733899.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T162739 WP_044650325.1 NZ_CP056145:280999-281274 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T162739 NZ_CP071992:1015010-1015192 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAACGCTATGGTGACTGATTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAAATTTCACAGGAGAATGATGAAGAAAAATTAAATCAACCTCATGATAACGAAGTTCGTT
CAGATTTTAAAAATAGTAAATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT162739 WP_004181489.1 NZ_CP056145:281271-281630 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT162739 NZ_CP071992:1014387-1014986 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGACAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTAGGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTGG
AAAATCATGCAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCAATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAGGGAAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGCAAACTTGACGTTAGTGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACCGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAGAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References