Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9296..9939 Replicon plasmid pRHBSTW-00916_2
Accession NZ_CP056133
Organism Enterobacter hormaechei strain RHBSTW-00916

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag HV326_RS22850 Protein ID WP_000754567.1
Coordinates 9523..9939 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag HV326_RS22845 Protein ID WP_023293777.1
Coordinates 9296..9526 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV326_RS22805 4485..4958 + 474 WP_045340523.1 YkgJ family cysteine cluster protein -
HV326_RS22810 5050..5280 + 231 WP_011977773.1 hypothetical protein -
HV326_RS22815 6172..6954 - 783 WP_004152340.1 site-specific integrase -
HV326_RS22820 6954..7286 - 333 WP_004152339.1 hypothetical protein -
HV326_RS22825 7293..7691 - 399 WP_004171440.1 hypothetical protein -
HV326_RS22830 7717..8046 - 330 WP_004152337.1 hypothetical protein -
HV326_RS22835 8074..8382 - 309 WP_004152336.1 hypothetical protein -
HV326_RS22840 8428..8634 - 207 WP_004153649.1 hypothetical protein -
HV326_RS22845 9296..9526 + 231 WP_023293777.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV326_RS22850 9523..9939 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
HV326_RS22855 10290..11258 - 969 WP_016151349.1 IS5 family transposase -
HV326_RS22860 11418..11681 + 264 WP_001295708.1 hypothetical protein -
HV326_RS22865 11875..12156 + 282 WP_023332908.1 helix-turn-helix domain-containing protein -
HV326_RS22870 12191..12760 + 570 WP_023332907.1 small heat shock protein sHSP20 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..76485 76485
- flank IS/Tn - - 10290..11213 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T162738 WP_000754567.1 NZ_CP056133:9523-9939 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T162738 NZ_CP071992:868981-869343 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGATAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGATAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT162738 WP_023293777.1 NZ_CP056133:9296-9526 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT162738 NZ_CP071992:868814-868984 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAACGAATCACATTTAATATCTAATTCCA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References