Detailed information of TA system TA162737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4676054..4676670
• Replicon: chromosome
• Accession: NZ_CP056132
• Organism: Enterobacter hormaechei strain RHBSTW-00916

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV326_RS22365
• Protein ID: WP_077266491.1
• Coordinates: 4676054..4676425 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV326_RS22370
• Protein ID: WP_015569912.1
• Coordinates: 4676428..4676670 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV326_RS22350	4673554..4674456	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HV326_RS22355	4674453..4675088	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV326_RS22360	4675085..4676014	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV326_RS22365	4676054..4676425	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV326_RS22370	4676428..4676670	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV326_RS22375	4676869..4677789	+	921	WP_048983078.1	alpha/beta hydrolase	-
HV326_RS22380	4677798..4678739	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV326_RS22385	4678784..4679221	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV326_RS22390	4679218..4680099	-	882	WP_003861949.1	virulence factor BrkB family protein	-
HV326_RS22395	4680093..4680692	-	600	WP_032649898.1	glucose-1-phosphatase	-
HV326_RS22400	4680811..4681611	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T162737 WP_077266491.1 NZ_CP056132:c4676425-4676054 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T162737 NZ_CP071992:857927-858136 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATACATGTTTCAGGTATTAAAACAACTGAACAAGAGAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTGGAATTTGACACTCCCGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTGCGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT162737 WP_015569912.1 NZ_CP056132:c4676670-4676428 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT162737 NZ_CP071992:857627-857920 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATCAATCGTATGATTGATGACGATGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTATTAAATTACTAGATCATCACATGAAGGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3