Detailed information of TA system TA162717

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 96187..96854
• Replicon: chromosome
• Accession: NZ_CP056132
• Organism: Enterobacter hormaechei strain RHBSTW-00916

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV326_RS00500
• Protein ID: WP_059443702.1
• Coordinates: 96187..96507 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV326_RS00505
• Protein ID: WP_059443453.1
• Coordinates: 96528..96854 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV326_RS00465	91422..92303	+	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
HV326_RS00470	92368..92667	-	300	WP_045335986.1	XRE family transcriptional regulator	-
HV326_RS00475	92664..93014	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV326_RS00480	93287..93539	-	253	Protein_95	DNA-binding protein	-
HV326_RS00485	93642..93983	-	342	WP_006812446.1	SymE family type I addiction module toxin	-
HV326_RS00490	94120..94296	+	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
HV326_RS00495	94336..95892	-	1557	WP_047050722.1	hypothetical protein	-
HV326_RS00500	96187..96507	-	321	WP_059443702.1	TA system toxin CbtA family protein	Toxin
HV326_RS00505	96528..96854	-	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV326_RS00510	96867..97337	-	471	WP_015703840.1	DNA repair protein RadC	-
HV326_RS00515	97406..98227	-	822	WP_015703839.1	DUF945 domain-containing protein	-
HV326_RS00520	98320..99330	-	1011	WP_063153702.1	hypothetical protein	-
HV326_RS00525	99392..100081	-	690	WP_015703837.1	WYL domain-containing protein	-
HV326_RS00530	100273..101166	-	894	WP_063153701.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	89910..111142	21232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-99)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12048.92 Da
• Isoelectric Point: 6.2120

>T162717 WP_059443702.1 NZ_CP056132:c96507-96187 [Enterobacter hormaechei]
MNTQPATTPQAAKPCLSPLAVWKMLLTILLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAINFLVERYELVRIDQKGF
SVQDQEPWLTSMDVHRARFKLNVARL

Nucleotide

• Length: 321 bp

>T162717 NZ_CP071975:c904225-903899 [Helicobacter pylori]
GTGTTGAAGCTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAGAAAGAACCGCTAGATCCACGATTTCAAGATCATGCCTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATCAAAGCCGACATTTTGCTTGTGTATTTAGTGAAAGATGATGAACTGATTTTGTTAAGG
TTAGGCAGTCATAACACGCTACTTTGTAAATCACCCACACCCCTTATAACGCTTAAACTAATCGCCCACCAACTACCCCA
TTTTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12277.99 Da
• Isoelectric Point: 6.3620

>AT162717 WP_059443453.1 NZ_CP056132:c96854-96528 [Enterobacter hormaechei]
MSLIPAHEWGLQREIVPRFGARLVQEGNRLHYLADRASLMGNFSDTECFKLNDAFPHFISQMESMLTTGELIPCHHHCVT
LYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 327 bp

>AT162717 NZ_CP071975:c904583-904206 [Helicobacter pylori]
ATGCCAAACACCACCGCAGAAAAAGACTACACAAAATACAGCCAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGAAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGCTGTTTT
AGGCATGAGATCCCAAACATTCAAACGCAACAAGCCATGAGAGATGCGTATAACAAAGAGACAGATTTGTTCGTTGAGGA
TTTTTCTAGTTATAGCAATGAGAGAAAAAAGGCTTTAGGTGTTGAAGCTCAATCTTAA