Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4042997..4043573 Replicon chromosome
Accession NZ_CP056129
Organism Enterobacter roggenkampii strain RHBSTW-00925

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag HV328_RS19380 Protein ID WP_025912099.1
Coordinates 4043286..4043573 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag HV328_RS19375 Protein ID WP_023293359.1
Coordinates 4042997..4043299 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV328_RS19350 4039105..4039650 + 546 WP_047746519.1 porin -
HV328_RS19355 4039650..4039970 + 321 WP_008501314.1 hypothetical protein -
HV328_RS19360 4040015..4041385 - 1371 WP_181518993.1 NAD-dependent succinate-semialdehyde dehydrogenase -
HV328_RS19365 4041537..4042280 + 744 WP_046092376.1 AraC family transcriptional regulator -
HV328_RS19370 4042328..4042966 + 639 WP_048962264.1 LysE family translocator -
HV328_RS19375 4042997..4043299 - 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
HV328_RS19380 4043286..4043573 - 288 WP_025912099.1 BrnT family toxin Toxin
HV328_RS19385 4043743..4045014 + 1272 WP_025912097.1 DUF445 domain-containing protein -
HV328_RS19390 4045011..4046087 - 1077 WP_047746527.1 DUF2955 domain-containing protein -
HV328_RS19395 4046077..4047144 - 1068 WP_025912094.1 HlyD family secretion protein -
HV328_RS19400 4047141..4047611 - 471 WP_047746530.1 MarR family transcriptional regulator -
HV328_RS19405 4047758..4048453 - 696 WP_045371542.1 winged helix-turn-helix domain-containing protein -
HV328_RS19410 4048446..4048562 - 117 Protein_3795 amino acid-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T162711 WP_025912099.1 NZ_CP056129:c4043573-4043286 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T162711 NZ_CP071960:32266-32523 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT162711 WP_023293359.1 NZ_CP056129:c4043299-4042997 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT162711 NZ_CP071960:32004-32273 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References