Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2900787..2901584 Replicon chromosome
Accession NZ_CP056129
Organism Enterobacter roggenkampii strain RHBSTW-00925

Toxin (Protein)


Gene name KacT Uniprot ID A0A155TD66
Locus tag HV328_RS13960 Protein ID WP_032651255.1
Coordinates 2900787..2901308 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W7PG43
Locus tag HV328_RS13965 Protein ID WP_008499829.1
Coordinates 2901315..2901584 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV328_RS13930 2896370..2897059 + 690 WP_047745127.1 pyrimidine utilization protein B -
HV328_RS13935 2897071..2897457 + 387 WP_047745126.1 pyrimidine utilization protein C -
HV328_RS13940 2897465..2898265 + 801 WP_021240807.1 pyrimidine utilization protein D -
HV328_RS13945 2898275..2898865 + 591 WP_047745125.1 malonic semialdehyde reductase -
HV328_RS13950 2898875..2899369 + 495 WP_023292823.1 pyrimidine utilization flavin reductase protein F -
HV328_RS13955 2899391..2900713 + 1323 WP_008499826.1 pyrimidine utilization transport protein G -
HV328_RS13960 2900787..2901308 - 522 WP_032651255.1 GNAT family N-acetyltransferase Toxin
HV328_RS13965 2901315..2901584 - 270 WP_008499829.1 DUF1778 domain-containing protein Antitoxin
HV328_RS13970 2901646..2902563 - 918 Protein_2734 DMT family transporter -
HV328_RS13975 2902682..2902852 - 171 WP_013097201.1 general stress protein -
HV328_RS13980 2903244..2903840 + 597 WP_008499831.1 NAD(P)H:quinone oxidoreductase -
HV328_RS13985 2903861..2904088 + 228 WP_008499832.1 YccJ family protein -
HV328_RS13990 2904124..2905365 - 1242 WP_023343731.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
HV328_RS13995 2905518..2905961 + 444 WP_047745123.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19714.46 Da        Isoelectric Point: 7.0497

>T162708 WP_032651255.1 NZ_CP056129:c2901308-2900787 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Download         Length: 522 bp

>T162708 NZ_CP071959:50391-50648 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10262.72 Da        Isoelectric Point: 5.8836

>AT162708 WP_008499829.1 NZ_CP056129:c2901584-2901315 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT162708 NZ_CP071959:50129-50398 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155TD66

Antitoxin

Source ID Structure
AlphaFold DB W7PG43

References