Detailed information of TA system TA162696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 46656..47317
• Replicon: chromosome
• Accession: NZ_CP056129
• Organism: Enterobacter roggenkampii strain RHBSTW-00925

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV328_RS00240
• Protein ID: WP_023293716.1
• Coordinates: 46919..47317 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B3V1Y0
• Locus tag: HV328_RS00235
• Protein ID: WP_045414808.1
• Coordinates: 46656..46922 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV328_RS00205	42708..43022	-	315	WP_021242703.1	PTS sugar transporter subunit IIB	-
HV328_RS00210	43316..44266	+	951	WP_023293714.1	LacI family transcriptional regulator	-
HV328_RS00215	44328..44624	-	297	WP_021242704.1	hypothetical protein	-
HV328_RS00220	44766..44906	+	141	Protein_43	N-acetyltransferase	-
HV328_RS00230	46251..46526	+	276	Protein_45	GNAT family N-acetyltransferase	-
HV328_RS00235	46656..46922	+	267	WP_045414808.1	virulence protein	Antitoxin
HV328_RS00240	46919..47317	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV328_RS00245	47457..48257	+	801	WP_047745055.1	lipoprotein NlpA	-
HV328_RS00250	48381..49160	+	780	WP_047745054.1	MBL fold metallo-hydrolase	-
HV328_RS00255	49187..50140	+	954	WP_045414812.1	helix-turn-helix domain-containing protein	-
HV328_RS00260	50323..52020	+	1698	WP_181519314.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T162696 WP_023293716.1 NZ_CP056129:46919-47317 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T162696 NZ_CP071955:1535560-1535979 [Xanthomonas fragariae]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAACCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGCGGAAACCCTCTACCTGTCCAGCGTGACGCTTGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAAAAAGGCCTGGGCGAAGCCCTGGACGGATTGCTCGAACTGTTCAGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCAATTATGCCGAGCTGGCGGTGAAGGCCCGCACAGCCGGGAAGGGTTTTCCAACACCTGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTCGAAGCGGCCGGGCTTACTGTTATCA
ATCCTTGGAATAATCAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9981.32 Da
• Isoelectric Point: 4.6310

>AT162696 WP_045414808.1 NZ_CP056129:46656-46922 [Enterobacter roggenkampii]
MERTAKLFKKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT162696 NZ_CP071955:1535309-1535563 [Xanthomonas fragariae]
ATGGCAATCTTGACAGTGCGGAATGTGCCCGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTCCGCGAGATTCTGGAGAGTGCAGTTAAGTCAGAAAAGCGCGTCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCAAGTAGGGCTGACAAACGATGATTTCGCAGTGTTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B3V1Y0