Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 12187..12923 Replicon plasmid pRHBSTW-01009_2
Accession NZ_CP056127
Organism Enterobacter asburiae strain RHBSTW-01009

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV349_RS22645 Protein ID WP_003026803.1
Coordinates 12441..12923 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV349_RS22640 Protein ID WP_003026799.1
Coordinates 12187..12453 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV349_RS22625 9211..10206 + 996 WP_023311709.1 type 3 fimbria adhesin subunit MrkD -
HV349_RS22630 10220..10855 + 636 WP_000677445.1 type 3 fimbria minor subunit MrkF -
HV349_RS22635 10958..11926 - 969 WP_000654811.1 IS5 family transposase -
HV349_RS22640 12187..12453 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV349_RS22645 12441..12923 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV349_RS22650 13082..14614 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
HV349_RS22655 14736..15704 - 969 WP_181599869.1 IS5 family transposase -
HV349_RS22660 15864..16127 + 264 WP_001295708.1 hypothetical protein -
HV349_RS22665 16349..16630 + 282 WP_004118205.1 helix-turn-helix domain-containing protein -
HV349_RS22670 16665..17234 + 570 WP_000350436.1 small heat shock protein sHSP20 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-10 mrkA / mrkB / mrkC / mrkD / mrkF 1..70650 70650
- inside IScluster/Tn - - 10958..15704 4746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T162695 WP_003026803.1 NZ_CP056127:12441-12923 [Enterobacter asburiae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T162695 NZ_CP071955:c1388508-1388146 [Xanthomonas fragariae]
ATGCCTGAAAAGCCTCTGATATGGGTTGGATCAAGTAGACGTGACTTGGCAGAGCTACCTGAAGACGTTAAGGATACCTT
TGGATTTGCACTGTCCGAAGCTCAGAACGGACGCACCCATGTGGGAGCAAAGCTTATGGCTAAGGGATCACTGAAGGGTA
AGGGCATTTACGAGGTGGTGGACGACTATGATGGTGACACTTTTAGGGCCGTCTATACGGTGGAAATAGAGGATGCCATT
TATGCCTTGCACTGCTTCCAAAAGAAATCTAAGAACGGCAGAGAAACTCCAAAATCAGACATCGACTTGATCGAATCTCG
GTATCAAACCGCCTTATTGGATGCAAAAAAGGTAAAAATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT162695 WP_003026799.1 NZ_CP056127:12187-12453 [Enterobacter asburiae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT162695 NZ_CP071955:c1388146-1387829 [Xanthomonas fragariae]
ATGAGCATTGAAATCGAAGTCAGCAGCGGTAACATTTACTCAGATCTTGGCTATTCAAATGCCGAGGAAATGAAAATTAA
AGCTGCGCTTGCTGGAAAGATTAGCGCATTCATTGCGGACGCACATCTCACTCAGGCTCAAGCAGCTGCCCACATGGGCA
TCTCTCAAGCAAAAGTGTCCGAAATGCTCCGGGGTCGCTTTCGCGGAATCTCCGAGGCTAAGATGATGGAATGCCTTAAG
AGCTTTGGTCAGAACGTTACAATAAGCGTCACACCGGCTGAAGTTCCTGACGAGGCGGAGATTTCTGTTGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References