162650

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 84873..85398 Replicon plasmid pEC394-330266_128
Accession NZ_CP056115
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HWD38_RS25230 Protein ID WP_001159868.1
Coordinates 85093..85398 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HWD38_RS25225 Protein ID WP_000813634.1
Coordinates 84873..85091 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS25200 (HWD38_24905) 80152..81765 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
HWD38_RS25205 (HWD38_24910) 81796..82146 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
HWD38_RS25210 (HWD38_24915) 82143..82568 - 426 WP_000422741.1 transposase -
HWD38_RS25215 (HWD38_24920) 82660..83781 + 1122 WP_012783980.1 DUF3800 domain-containing protein -
HWD38_RS25220 (HWD38_24925) 83815..84303 - 489 WP_011254646.1 hypothetical protein -
HWD38_RS25225 (HWD38_24930) 84873..85091 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HWD38_RS25230 (HWD38_24935) 85093..85398 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HWD38_RS25235 (HWD38_24940) 85399..86205 + 807 WP_000016982.1 site-specific integrase -
HWD38_RS25240 (HWD38_24945) 86979..87734 + 756 WP_000852146.1 replication initiation protein RepE -
HWD38_RS25245 (HWD38_24950) 88322..89488 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..128537 128537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T162650 WP_001159868.1 NZ_CP056115:85093-85398 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T162650 NZ_CP071930:191789-192061 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT162650 WP_000813634.1 NZ_CP056115:84873-85091 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT162650 NZ_CP071930:191526-191789 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References