162649

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77747..78390 Replicon plasmid pEC394-330266_128
Accession NZ_CP056115
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HWD38_RS25180 Protein ID WP_001034046.1
Coordinates 77747..78163 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HWD38_RS25185 Protein ID WP_001261278.1
Coordinates 78160..78390 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS25165 (HWD38_24870) 72884..73300 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HWD38_RS25170 (HWD38_24875) 73297..73527 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
HWD38_RS25175 (HWD38_24880) 73908..77702 + 3795 WP_001144732.1 hypothetical protein -
HWD38_RS25180 (HWD38_24885) 77747..78163 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HWD38_RS25185 (HWD38_24890) 78160..78390 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HWD38_RS25190 (HWD38_24895) 78655..79155 + 501 WP_000528932.1 HEPN family nuclease -
HWD38_RS25195 (HWD38_24900) 79168..79941 + 774 WP_000905949.1 hypothetical protein -
HWD38_RS25200 (HWD38_24905) 80152..81765 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
HWD38_RS25205 (HWD38_24910) 81796..82146 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
HWD38_RS25210 (HWD38_24915) 82143..82568 - 426 WP_000422741.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..128537 128537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T162649 WP_001034046.1 NZ_CP056115:c78163-77747 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T162649 NZ_CP071930:c62284-62099 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCAAAAAA
TTTTAAAGGATGCGGGATTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT162649 WP_001261278.1 NZ_CP056115:c78390-78160 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT162649 NZ_CP071930:c62065-61634 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References