Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 72884..73527 Replicon plasmid pEC394-330266_128
Accession NZ_CP056115
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HWD38_RS25165 Protein ID WP_001034044.1
Coordinates 72884..73300 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HWD38_RS25170 Protein ID WP_001261286.1
Coordinates 73297..73527 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS25140 (HWD38_24850) 68729..68997 + 269 Protein_82 type II toxin-antitoxin system ParD family antitoxin -
HWD38_RS25145 68984..69252 + 269 Protein_83 type II toxin-antitoxin system RelE/ParE family toxin -
HWD38_RS25150 (HWD38_24855) 69286..69983 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
HWD38_RS25155 (HWD38_24860) 70237..71259 - 1023 WP_000361402.1 helicase UvrD -
HWD38_RS25160 (HWD38_24865) 71244..72809 - 1566 WP_001128474.1 AAA family ATPase -
HWD38_RS25165 (HWD38_24870) 72884..73300 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HWD38_RS25170 (HWD38_24875) 73297..73527 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HWD38_RS25175 (HWD38_24880) 73908..77702 + 3795 WP_001144732.1 hypothetical protein -
HWD38_RS25180 (HWD38_24885) 77747..78163 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
HWD38_RS25185 (HWD38_24890) 78160..78390 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..128537 128537
- flank IS/Tn - - 69480..69983 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T162648 WP_001034044.1 NZ_CP056115:c73300-72884 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T162648 NZ_CP071918:c1671327-1671148 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT162648 WP_001261286.1 NZ_CP056115:c73527-73297 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT162648 NZ_CP071918:c1671111-1670659 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References