162647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 65874..66433 Replicon plasmid pEC394-330266_128
Accession NZ_CP056115
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag HWD38_RS25120 Protein ID WP_000421272.1
Coordinates 66158..66433 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag HWD38_RS25115 Protein ID WP_001387467.1
Coordinates 65874..66158 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS25085 (HWD38_24795) 61337..61777 - 441 Protein_71 zinc-binding dehydrogenase -
HWD38_RS25090 (HWD38_24800) 61751..61945 + 195 WP_000796505.1 hypothetical protein -
HWD38_RS25095 (HWD38_24805) 62178..63068 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
HWD38_RS25100 (HWD38_24810) 63093..63473 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
HWD38_RS25105 (HWD38_24815) 63506..64471 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
HWD38_RS25110 (HWD38_24820) 64517..65269 - 753 WP_000562172.1 IclR family transcriptional regulator -
HWD38_RS25115 (HWD38_24825) 65874..66158 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
HWD38_RS25120 (HWD38_24830) 66158..66433 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HWD38_RS25125 (HWD38_24835) 66539..66742 + 204 Protein_79 hypothetical protein -
HWD38_RS25130 (HWD38_24840) 66867..66995 - 129 Protein_80 IS481 family transposase -
HWD38_RS25135 (HWD38_24845) 67028..68197 - 1170 WP_001072355.1 hypothetical protein -
HWD38_RS25140 (HWD38_24850) 68729..68997 + 269 Protein_82 type II toxin-antitoxin system ParD family antitoxin -
HWD38_RS25145 68984..69252 + 269 Protein_83 type II toxin-antitoxin system RelE/ParE family toxin -
HWD38_RS25150 (HWD38_24855) 69286..69983 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
HWD38_RS25155 (HWD38_24860) 70237..71259 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..128537 128537
- flank IS/Tn - - 69480..69983 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T162647 WP_000421272.1 NZ_CP056115:66158-66433 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T162647 NZ_CP071918:955286-955540 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT162647 WP_001387467.1 NZ_CP056115:65874-66158 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT162647 NZ_CP071918:955042-955284 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References