162638

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3558285..3558861 Replicon chromosome
Accession NZ_CP056114
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag HWD38_RS17100 Protein ID WP_001295743.1
Coordinates 3558285..3558572 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag HWD38_RS17105 Protein ID WP_000063148.1
Coordinates 3558559..3558861 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS17085 (3553945) 3553945..3555249 - 1305 WP_000535012.1 restriction endonuclease subunit S -
HWD38_RS17090 (3555239) 3555239..3556858 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
HWD38_RS17095 (3556922) 3556922..3558088 - 1167 WP_000800831.1 restriction endonuclease -
HWD38_RS17100 (3558285) 3558285..3558572 + 288 WP_001295743.1 BrnT family toxin Toxin
HWD38_RS17105 (3558559) 3558559..3558861 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
HWD38_RS17110 (3558895) 3558895..3559851 - 957 WP_001295745.1 GTPase -
HWD38_RS17115 (3559862) 3559862..3560065 - 204 WP_000467859.1 YbdD/YjiX family protein -
HWD38_RS17120 (3560115) 3560115..3562265 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T162638 WP_001295743.1 NZ_CP056114:3558285-3558572 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T162638 NZ_CP071914:c1157810-1157397 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT162638 WP_000063148.1 NZ_CP056114:3558559-3558861 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT162638 NZ_CP071914:c1158030-1157797 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTTCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References