Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3477230..3478065 Replicon chromosome
Accession NZ_CP056114
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag HWD38_RS16710 Protein ID WP_000854759.1
Coordinates 3477688..3478065 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HWD38_RS16705 Protein ID WP_001295723.1
Coordinates 3477230..3477598 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS16680 (3473232) 3473232..3473705 + 474 WP_001350782.1 antirestriction protein -
HWD38_RS16685 (3473721) 3473721..3474197 + 477 WP_001186775.1 RadC family protein -
HWD38_RS16690 (3474260) 3474260..3474514 + 255 WP_023281719.1 DUF987 domain-containing protein -
HWD38_RS16695 (3474655) 3474655..3476196 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
HWD38_RS16700 (3476211) 3476211..3476957 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
HWD38_RS16705 (3477230) 3477230..3477598 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HWD38_RS16710 (3477688) 3477688..3478065 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
HWD38_RS16715 (3478062) 3478062..3478550 + 489 WP_000761690.1 DUF5983 family protein -
HWD38_RS16720 (3478567) 3478567..3478743 + 177 WP_000839286.1 DUF957 domain-containing protein -
HWD38_RS16725 (3478849) 3478849..3478998 + 150 Protein_3271 hypothetical protein -
HWD38_RS16730 (3479365) 3479365..3479541 + 177 Protein_3272 helix-turn-helix domain-containing protein -
HWD38_RS16735 (3480756) 3480756..3481952 + 1197 WP_235692174.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T162637 WP_000854759.1 NZ_CP056114:3477688-3478065 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T162637 NZ_CP071903:1785759-1786013 [Mucilaginibacter rubeus]
GTGAAGATAATTTTCCAAAACCCTGCATGGGAAGATTACCTTTATTGGCAAACGACAGATAAATCTATTTTAAAGAAGAT
CAACTCTCTTATCAAAGAAATAGAACGAACTCCATATGAAGGAAGTGGAAAGCCCGAACCTTTAAAACATAATTTAGCTG
GTTGGTGGTCGCGCCGGATAAATCTCGAACACAGGCTAATTTACAGAGTAGAAGCAGACGCGATATTTATTCTGCAATGC
AGATATCATTATTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT162637 WP_001295723.1 NZ_CP056114:3477230-3477598 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT162637 NZ_CP071903:1785508-1785762 [Mucilaginibacter rubeus]
ATGGAGATCACAACTTACACTGCCTTCAGGCAGCAACTAAAATCTTTTTTAGATAAGGTGCGGAATGATCATAAGCCTTT
GGTAGTGACAAGTGCCAATGCCGAAGATATTGTTGTGATATCGAAAGCTGATTACGAAAGCATGGAGGAAACTTTCTATC
TTTTAAAAAGCCCTAAAAATGCAGAAAGATTACTTCATGGTATCCAAGAATATGAAAAAGGTTTAGGCAAGGAAAGAAAG
CTACTTGAAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References