Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3274644..3275141 Replicon chromosome
Accession NZ_CP056114
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HWD38_RS15670 Protein ID WP_001173343.1
Coordinates 3274968..3275141 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HWD38_RS15665 Protein ID WP_001312331.1
Coordinates 3274644..3274940 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS15655 (3270001) 3270001..3273930 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
HWD38_RS15660 (3274144) 3274144..3274416 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HWD38_RS15665 (3274644) 3274644..3274940 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HWD38_RS15670 (3274968) 3274968..3275141 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HWD38_RS15675 (3275260) 3275260..3276777 - 1518 WP_000003806.1 lysine--tRNA ligase -
HWD38_RS15680 (3277014) 3277014..3278471 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3259054..3283995 24941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162635 WP_001173343.1 NZ_CP056114:3274968-3275141 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162635 NZ_CP071900:c54078-53896 [Pseudomonas stutzeri]
ATGAAGTACAGCGAGTTTCGGCGGTGGTTGAAGGCTCAAGGCGTTAGCTTCGAGCCAGCCAAGGGAAGCCACTTCAAGGC
ATACTTTGGTGACCGCCAGACCATCGTCCCCGACCATGGATCCAAGGAAATTGGCGAAGGGTTGAGGAAGAAGATTCTGA
AAGACCTGGGGCTCAAGGAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT162635 WP_001312331.1 NZ_CP056114:3274644-3274940 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162635 NZ_CP071900:c53859-53437 [Pseudomonas stutzeri]
ATGTTTGATTACCCAGTAATCGTGCACCAGGAAGATGGCTGCGTGTGGATCAGCTCTGACGATGTGCCGGAGCTGAACAG
CGCCGGTGACAACGAGGCCGAGGCGTTGCTGGATGCGGTGGACGGGCTGGAGTCGGCCCTGTCTCTCTACGTTGACCAAC
GCCGCGCGGTACCGCTGCCTTCGCCGGCAAAGCCTGGCCAGCCCGTGGTGCGCCTGCCCGCGCTGACTGCCGCCAAGGTC
GCGCTGTGGAACGCAATGTGTGCCCAAGGTGTTGGCAAGGCTGAGATGGCGCGCCGGTTGGGTGTAAATCGCCCGCAGGT
GGATCGGCTGGTGGATCTGCTTCACCGCTCGAAGATCGAACAGGTGGAGCATGCCCTCGCCATCCTGGGGCAACGACTGG
AGATCTCGGTGATCGCAGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References