Detailed information of TA system TA162629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 1859961..1861242
• Replicon: chromosome
• Accession: NZ_CP056114
• Organism: Escherichia coli strain Ec394-330266

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: A0A1X3JHM4
• Locus tag: HWD38_RS08980
• Protein ID: WP_001114681.1
• Coordinates: 1860349..1861242 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: V0YCI1
• Locus tag: HWD38_RS08975
• Protein ID: WP_000208383.1
• Coordinates: 1859961..1860356 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWD38_RS08930 (1855455)	1855455..1855940	+	486	WP_000849566.1	antirestriction protein	-
HWD38_RS08935 (1855956)	1855956..1856432	+	477	WP_001186726.1	RadC family protein	-
HWD38_RS08940 (1856495)	1856495..1856716	+	222	WP_000692329.1	DUF987 domain-containing protein	-
HWD38_RS08945 (1856735)	1856735..1857379	+	645	WP_000094916.1	hypothetical protein	-
HWD38_RS08950 (1857429)	1857429..1857809	+	381	WP_001285598.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HWD38_RS08955 (1857886)	1857886..1858263	+	378	WP_000854689.1	TA system toxin CbtA family protein	-
HWD38_RS08960 (1858260)	1858260..1858748	+	489	WP_000761699.1	DUF5983 family protein	-
HWD38_RS08965 (1858765)	1858765..1858962	+	198	WP_000839293.1	DUF957 domain-containing protein	-
HWD38_RS08970 (1859047)	1859047..1859892	+	846	WP_023281696.1	DUF4942 domain-containing protein	-
HWD38_RS08975 (1859961)	1859961..1860356	+	396	WP_000208383.1	DUF6088 family protein	Antitoxin
HWD38_RS08980 (1860349)	1860349..1861242	+	894	WP_001114681.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HWD38_RS08985 (1861713)	1861713..1861883	+	171	Protein_1766	IS110 family transposase	-
HWD38_RS08990 (1862232)	1862232..1863731	+	1500	WP_000174401.1	IS21-like element ISEc10 family transposase	-
HWD38_RS08995 (1863728)	1863728..1864483	+	756	Protein_1768	IS21-like element ISEc10 family helper ATPase IstB	-
HWD38_RS09000 (1865109)	1865109..1866092	+	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-292)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 34298.40 Da
• Isoelectric Point: 5.9850

>T162629 WP_001114681.1 NZ_CP056114:1860349-1861242 [Escherichia coli]
MPELTSLFPDVADALDIESVAIVEKDYFVVDLLRLLKEIKPETHTLVFAGSTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPITAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDYPVHKLRFQRFVNPMVFGNTQIN

Nucleotide

• Length: 894 bp

>T162629 NZ_CP071891:c2386928-2386776 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14608.03 Da
• Isoelectric Point: 11.3150

>AT162629 WP_000208383.1 NZ_CP056114:1859961-1860356 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITE
ALERLNIRYRLAGATECYNKGQSTQIPVSLGIKTPSRFKRVLIVGKSKINA

Nucleotide

• Length: 396 bp

>AT162629 NZ_CP071891:c2386712-2386578 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X3JHM4

Antitoxin

• AlphaFold DB: V0YCI1