Detailed information of TA system TA162628

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1857429..1858263
• Replicon: chromosome
• Accession: NZ_CP056114
• Organism: Escherichia coli strain Ec394-330266

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: HWD38_RS08955
• Protein ID: WP_000854689.1
• Coordinates: 1857886..1858263 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1X3JHN3
• Locus tag: HWD38_RS08950
• Protein ID: WP_001285598.1
• Coordinates: 1857429..1857809 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWD38_RS08915 (1853712)	1853712..1854167	+	456	WP_000581502.1	IrmA family protein	-
HWD38_RS08920 (1854272)	1854272..1854481	+	210	WP_032142237.1	DUF905 family protein	-
HWD38_RS08925 (1854582)	1854582..1855400	+	819	WP_001234620.1	DUF932 domain-containing protein	-
HWD38_RS08930 (1855455)	1855455..1855940	+	486	WP_000849566.1	antirestriction protein	-
HWD38_RS08935 (1855956)	1855956..1856432	+	477	WP_001186726.1	RadC family protein	-
HWD38_RS08940 (1856495)	1856495..1856716	+	222	WP_000692329.1	DUF987 domain-containing protein	-
HWD38_RS08945 (1856735)	1856735..1857379	+	645	WP_000094916.1	hypothetical protein	-
HWD38_RS08950 (1857429)	1857429..1857809	+	381	WP_001285598.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWD38_RS08955 (1857886)	1857886..1858263	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
HWD38_RS08960 (1858260)	1858260..1858748	+	489	WP_000761699.1	DUF5983 family protein	-
HWD38_RS08965 (1858765)	1858765..1858962	+	198	WP_000839293.1	DUF957 domain-containing protein	-
HWD38_RS08970 (1859047)	1859047..1859892	+	846	WP_023281696.1	DUF4942 domain-containing protein	-
HWD38_RS08975 (1859961)	1859961..1860356	+	396	WP_000208383.1	DUF6088 family protein	-
HWD38_RS08980 (1860349)	1860349..1861242	+	894	WP_001114681.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HWD38_RS08985 (1861713)	1861713..1861883	+	171	Protein_1766	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T162628 WP_000854689.1 NZ_CP056114:1857886-1858263 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T162628 NZ_CP071891:c2386712-2386578 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13927.72 Da
• Isoelectric Point: 4.7959

>AT162628 WP_001285598.1 NZ_CP056114:1857429-1857809 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT162628 NZ_CP071891:c2386928-2386776 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1X3JHN3