Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 776954..777785 Replicon chromosome
Accession NZ_CP056114
Organism Escherichia coli strain Ec394-330266

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag HWD38_RS03960 Protein ID WP_000854815.1
Coordinates 777411..777785 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag HWD38_RS03955 Protein ID WP_001280918.1
Coordinates 776954..777322 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWD38_RS03910 (772043) 772043..772789 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
HWD38_RS03915 (772872) 772872..773222 + 351 Protein_772 hypothetical protein -
HWD38_RS03920 (773238) 773238..773648 + 411 WP_000846703.1 hypothetical protein -
HWD38_RS03925 (773869) 773869..774687 + 819 WP_001542275.1 DUF932 domain-containing protein -
HWD38_RS03930 (774687) 774687..774932 + 246 WP_001164966.1 hypothetical protein -
HWD38_RS03935 (775026) 775026..775499 + 474 WP_001542276.1 antirestriction protein -
HWD38_RS03940 (775515) 775515..775991 + 477 WP_001186200.1 RadC family protein -
HWD38_RS03945 (776054) 776054..776275 + 222 WP_000692345.1 DUF987 domain-containing protein -
HWD38_RS03950 (776294) 776294..776938 + 645 WP_000086752.1 hypothetical protein -
HWD38_RS03955 (776954) 776954..777322 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HWD38_RS03960 (777411) 777411..777785 + 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HWD38_RS03965 (777782) 777782..777976 + 195 WP_000988600.1 DUF5983 family protein -
HWD38_RS03970 (778022) 778022..778102 + 81 Protein_783 hypothetical protein -
HWD38_RS03975 (778391) 778391..778471 - 81 WP_023441679.1 hypothetical protein -
HWD38_RS03980 (778450) 778450..778773 + 324 WP_225469267.1 EutP/PduV family microcompartment system protein -
HWD38_RS03985 (778874) 778874..779203 - 330 WP_000450409.1 DUF496 family protein -
HWD38_RS03990 (779375) 779375..780433 - 1059 WP_001200889.1 FUSC family protein -
HWD38_RS03995 (780631) 780631..781104 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
HWD38_RS04000 (781223) 781223..782389 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T162625 WP_000854815.1 NZ_CP056114:777411-777785 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T162625 NZ_CP071889:1021671-1021934 [Halomonas sp. XH26]
GTGACATGGACGTTGGTTTACACCAAACAAGCCCAGAAAGACGCAAAGAAGCTGGCTTCAAGCGGCCTCAAGCCAAAAGC
CCAAGAGTTGTTGGCCCTAGTTGCAGAAGATCCGTACCGCAAGCCACCCCCGTTTGAGAAGCCCATAGGTGATCTTGCGG
GCGCCTATTCACGCCGCATTAATATTCAGCATCGTCTGGTCTACCAAGTGCTAGAAGACGAGCAAGTAGTGAAAGTCCTC
AGGCTATGGAGCCACTACGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT162625 WP_001280918.1 NZ_CP056114:776954-777322 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT162625 NZ_CP071889:1021432-1021674 [Halomonas sp. XH26]
ATGACCGGAATCACAGCAACGGAGGCGCGGAGCAACCTTTATCGGTTGATTGACGAGACTGCCGAGTCCCACCAACCCAT
CGTCATCATGGGTAAGCGGAACAAAGCCGTCCTGGTTTCCGAGGAAGACTGGTCCGCCATTCAGGAAACGCTTTACCTGC
TCTCCGTACCGGGTATGCGGGAGTCTATTCGTGAGGGAATGGATACCTCCGTGGATGAATGCGATGAGGAGCTGAACTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References