Detailed information of TA system TA162614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2084570..2085988
• Replicon: chromosome
• Accession: NZ_CP056087
• Organism: Pseudomonas argentinensis strain SA190

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: SA190iCDA_RS09230
• Protein ID: WP_070886660.1
• Coordinates: 2084570..2085139 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: SA190iCDA_RS09235
• Protein ID: WP_070886661.1
• Coordinates: 2085143..2085988 (+)
• Length: 282 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA190iCDA_RS09220	2080202..2080540	-	339	WP_070886658.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
SA190iCDA_RS09225	2080855..2084331	-	3477	WP_070886659.1	hybrid sensor histidine kinase/response regulator	-
SA190iCDA_RS09230	2084570..2085139	+	570	WP_070886660.1	GNAT family protein	Toxin
SA190iCDA_RS09235	2085143..2085988	+	846	WP_070886661.1	AraC family transcriptional regulator	Antitoxin
SA190iCDA_RS09240	2086313..2087023	+	711	WP_070886662.1	AzlC family ABC transporter permease	-
SA190iCDA_RS09245	2087023..2087352	+	330	WP_070886663.1	AzlD domain-containing protein	-
SA190iCDA_RS09250	2087487..2088104	+	618	WP_070886664.1	glutathione S-transferase	-
SA190iCDA_RS09255	2088143..2090209	+	2067	WP_070886665.1	pyridoxamine 5'-phosphate oxidase family protein	-
SA190iCDA_RS09260	2090241..2090720	+	480	WP_205881604.1	DUF1348 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (194-271)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21577.00 Da
• Isoelectric Point: 8.4936

>T162614 WP_070886660.1 NZ_CP056087:2084570-2085139 [Pseudomonas argentinensis]
MFKPQLITLQRGALRIEPLLDGDIPALVSLAEANREELLYMSGTQRLDWYRLALQEQREERALPLVIRLADQVVGTTRFA
DFMPTLPAVEIGWTWLDAAQHGTGLNNMVKYLMLRHAFESWQMVRVQLKTAASNLRAQRAIEKLGAIREGLLRNHRRLAD
GRLDDTVLYSITDQEWPLVKAGLEARFGV

Nucleotide

• Length: 570 bp

>T162614 NZ_CP071879:c79751-79497 [Mucilaginibacter rubeus]
GTGAAGATAATTTTCCAAAACCCTGCATGGGAAGATTACCTTTATTGGCAAACGACAGATAAATCTATTTTAAAGAAGAT
CAACTCTCTTATCAAAGAAATAGAACGAACTCCATATGAAGGAAGTGGAAAGCCCGAACCTTTAAAACATAATTTAGCTG
GTTGGTGGTCGCGCCGGATAAATCTCGAACACAGGCTAATTTACAGAGTAGAAGCAGACGCGATATTTATTCTGCAATGC
AGATATCATTATTGA

Antitoxin

Protein

• Length: 282 a.a.
• Molecular weight: 31071.59 Da
• Isoelectric Point: 10.2714

>AT162614 WP_070886661.1 NZ_CP056087:2085143-2085988 [Pseudomonas argentinensis]
MPTGERIRFWESPALAGVELLQARYIKQRFTPHVHEGFVFTVIEGGAQRFRHRGADHLAPIGSMVLINPDEVHTGSAAHE
QGWFYRAFYPAPAQVSGVLDELGLAQRGLPSFAASVLQDVQLHQAFGQLHRLLDSDASVLQQQTAWRETLLLLLFQRHAG
VRQAPTPGQEPRAVSLAKELLAARLGEPPTLEALAAAVNLSPFHFARVFRRATGLPPHAWLQQRRLERARALLRNGCAPL
SVALQLGFADQSHLTRQFKQVYGVGPGEYRKACHRSPVACG

Nucleotide

• Length: 846 bp

>AT162614 NZ_CP071879:c80002-79748 [Mucilaginibacter rubeus]
ATGGAGATCACAACTTACACTGCCTTCAGGCAGCAACTAAAATCTTTTTTAGATAAGGTGCGGAATGATCATAAGCCTTT
GGTAGTGACAAGTGCCAATGCCGAAGATATTGTTGTGATATCGAAAGCTGATTACGAAAGCATGGAGGAAACTTTCTATC
TTTTAAAAAGCCCTAAAAATGCAGAAAGATTACTTCATGGTATCCAAGAATATGAAAAAGGTTTAGGCAAGGAAAGAAAG
CTACTTGAAGAGTGA