Detailed information of TA system TA162612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2789909..2790570
• Replicon: chromosome
• Accession: NZ_CP056085
• Organism: Ralstonia solanacearum strain FJAT91-F8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: HWE47_RS12900
• Protein ID: WP_016727753.1
• Coordinates: 2790157..2790570 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: HWE47_RS12895
• Protein ID: WP_019717749.1
• Coordinates: 2789909..2790160 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWE47_RS12865	2785403..2785552	+	150	WP_155738951.1	hypothetical protein	-
HWE47_RS12870	2785613..2786227	-	615	WP_157698853.1	hypothetical protein	-
HWE47_RS12875	2786928..2787644	+	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
HWE47_RS12880	2788319..2788534	-	216	WP_071624182.1	glycine zipper 2TM domain-containing protein	-
HWE47_RS12885	2788931..2789146	+	216	WP_071615547.1	hypothetical protein	-
HWE47_RS12890	2789300..2789532	-	233	Protein_2518	hypothetical protein	-
HWE47_RS12895	2789909..2790160	+	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HWE47_RS12900	2790157..2790570	+	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWE47_RS12905	2790642..2791664	-	1023	WP_176599528.1	DNA cytosine methyltransferase	-
HWE47_RS12910	2791804..2792097	+	294	WP_020371973.1	hypothetical protein	-
HWE47_RS12915	2792485..2793330	+	846	WP_157698852.1	hypothetical protein	-
HWE47_RS12920	2793564..2794208	-	645	WP_086706230.1	hypothetical protein	-
HWE47_RS12925	2794284..2794436	-	153	WP_157698851.1	hypothetical protein	-
HWE47_RS12930	2794445..2795008	-	564	Protein_2526	hypothetical protein	-
HWE47_RS12935	2794961..2795248	+	288	WP_086706365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2786021..2806673	20652
	-	flank	IS/Tn	-	-	2784441..2785406	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T162612 WP_016727753.1 NZ_CP056085:2790157-2790570 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T162612 NZ_CP071878:c4747826-4747572 [Mucilaginibacter gossypii]
GTGAAGATAATTTTCCAAAACCCTGCATGGGAAGATTACCTTTATTGGCAAACGACAGATAAATCTATTTTAAAGAAGAT
CAACTCTCTTATCAAAGAAATAGAACGAACTCCATATGAAGGAAGTGGAAAGCCCGAACCGTTAAAACATAATTTAGCTG
GTTGGTGGTCGCGCCGGATAAATCTCGAACACAGGCTAATTTACAGAGTAGAAGCAGACGCGATATTTATTCTGCAATGC
AGATATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT162612 WP_019717749.1 NZ_CP056085:2789909-2790160 [Ralstonia solanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT162612 NZ_CP071878:c4748077-4747823 [Mucilaginibacter gossypii]
ATGGAGATCACAACTTACACTGCCTTCAGGCAGCAACTAAAATCTTTTTTAGATAAGGTGCGGAATGATCATAAGCCTTT
GGTAGTGACAAGTGCCAATGCCGAAGATATTGTTGTGATATCGAAAGCTGATTACGAAAGCATGGAGGAAACTTTCTATC
TTTTAAAAAGCCCTAAAAATGCAGAAAGATTACTTCATGGTATCCAGGAATATGAAAAAGGTTTAGGCAAGGAAAGAAAG
CTACTTGAAGAGTGA