Detailed information of TA system TA162608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2790021..2790682
• Replicon: chromosome
• Accession: NZ_CP056083
• Organism: Ralstonia solanacearum strain FJAT91-F1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: HWE45_RS12910
• Protein ID: WP_016727753.1
• Coordinates: 2790269..2790682 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: HWE45_RS12905
• Protein ID: WP_019717749.1
• Coordinates: 2790021..2790272 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWE45_RS12875	2785515..2785664	+	150	WP_155738951.1	hypothetical protein	-
HWE45_RS12880	2785725..2786339	-	615	WP_157698853.1	hypothetical protein	-
HWE45_RS12885	2787040..2787756	+	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
HWE45_RS12890	2788431..2788646	-	216	WP_071624182.1	glycine zipper 2TM domain-containing protein	-
HWE45_RS12895	2789043..2789258	+	216	WP_071615547.1	hypothetical protein	-
HWE45_RS12900	2789412..2789644	-	233	Protein_2519	hypothetical protein	-
HWE45_RS12905	2790021..2790272	+	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HWE45_RS12910	2790269..2790682	+	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWE45_RS12915	2790754..2791776	-	1023	WP_086706232.1	DNA cytosine methyltransferase	-
HWE45_RS12920	2791916..2792209	+	294	WP_020371973.1	hypothetical protein	-
HWE45_RS12925	2792597..2793442	+	846	WP_157698852.1	hypothetical protein	-
HWE45_RS12930	2793676..2794320	-	645	WP_086706230.1	hypothetical protein	-
HWE45_RS12935	2794396..2794548	-	153	WP_157698851.1	hypothetical protein	-
HWE45_RS12940	2794557..2795120	-	564	Protein_2527	hypothetical protein	-
HWE45_RS12945	2795073..2795360	+	288	WP_086706365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2784553..2785518	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T162608 WP_016727753.1 NZ_CP056083:2790269-2790682 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T162608 NZ_CP071876:2219802-2220104 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
CTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCTGTGGGAGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT162608 WP_019717749.1 NZ_CP056083:2790021-2790272 [Ralstonia solanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT162608 NZ_CP071876:2220111-2220398 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA