Detailed information of TA system TA162605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1859510..1860068
• Replicon: chromosome
• Accession: NZ_CP056080
• Organism: Rothia nasimurium strain E1706032

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HWU94_RS08475
• Protein ID: WP_185173333.1
• Coordinates: 1859754..1860068 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HWU94_RS08470
• Protein ID: WP_185173332.1
• Coordinates: 1859510..1859743 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWU94_RS08435	1854548..1855087	+	540	WP_185173325.1	hypothetical protein	-
HWU94_RS08440	1855059..1855442	+	384	WP_185173326.1	hypothetical protein	-
HWU94_RS08445	1855448..1855789	-	342	WP_185173327.1	MGMT family protein	-
HWU94_RS08450	1855796..1856770	-	975	WP_185173328.1	GNAT family N-acetyltransferase	-
HWU94_RS08455	1856968..1857588	+	621	WP_185173329.1	GNAT family N-acetyltransferase	-
HWU94_RS08460	1857729..1859111	-	1383	WP_185173330.1	glycine--tRNA ligase	-
HWU94_RS08465	1859252..1859470	+	219	WP_185173331.1	RNA-binding S4 domain-containing protein	-
HWU94_RS08470	1859510..1859743	+	234	WP_185173332.1	ribbon-helix-helix domain-containing protein	Antitoxin
HWU94_RS08475	1859754..1860068	+	315	WP_185173333.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HWU94_RS08480	1860096..1860782	-	687	WP_185173334.1	dienelactone hydrolase family protein	-
HWU94_RS08485	1860813..1862039	-	1227	WP_185173335.1	homoserine O-acetyltransferase	-
HWU94_RS08490	1862052..1863401	-	1350	WP_185173336.1	PLP-dependent transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1839063..1860068	21005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11400.31 Da
• Isoelectric Point: 8.5381

>T162605 WP_185173333.1 NZ_CP056080:1859754-1860068 [Rothia nasimurium]
VREICLVHLDKTRPAVVLTREPTRFAMTKVTIAPITSTIKGLSSEVLLGPANGLDRECAISADNILTVRQDTLGRTIGYL
TEPQEKQLAQAIIFAFDLRAPLAR

Nucleotide

• Length: 315 bp

>T162605 NZ_CP071876:1194303-1194614 [Enterobacter hormaechei]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.36 Da
• Isoelectric Point: 4.0827

>AT162605 WP_185173332.1 NZ_CP056080:1859510-1859743 [Rothia nasimurium]
MTVQIAVRLPDDLVAFLDHEVEEGQARSRAEIVAEALEHERRRRAALVDAAILASLGPEDDLDELVDWASGTPSSQQ

Nucleotide

• Length: 234 bp

>AT162605 NZ_CP071876:1194611-1195030 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA