Detailed information of TA system TA162597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 13853..14229
• Replicon: plasmid pMS1170_2
• Accession: NZ_CP056079
• Organism: Escherichia coli strain MS1170

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: HED12_RS23240
• Protein ID: WP_000969988.1
• Coordinates: 14047..14229 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HED12_RS23235
• Protein ID: WP_001324224.1
• Coordinates: 13853..14050 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HED12_RS23215	9113..9349	+	237	WP_001323890.1	colicin V immunity protein	-
HED12_RS23220	9327..9638	+	312	WP_001259758.1	colicin V	-
HED12_RS23225	9808..11922	-	2115	WP_202791759.1	peptidase domain-containing ABC transporter	-
HED12_RS23230	11897..13171	-	1275	WP_000489608.1	HlyD family secretion protein	-
HED12_RS23235	13853..14050	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
HED12_RS23240	14047..14229	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HED12_RS23245	14328..14636	+	309	WP_000377483.1	hypothetical protein	-
HED12_RS23250	15196..16230	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB	1..134406	134406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-58)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T162597 WP_000969988.1 NZ_CP056079:14047-14229 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T162597 NZ_CP071871:998999-999757 [Streptococcus pneumoniae]
GTGGAAATCCAAGATTATACTGACAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTTCAGCAA
GAATATGGCAAAGACAGTGTTGAATACACCAAATATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
GGGGATATGAAATACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATTAACCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAGGTTGTGTATATGATGCAA
AAGAAAATACAACTTCAGCGGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAAGTAGAGAAGGATATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT162597 WP_001324224.1 NZ_CP056079:13853-14050 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT162597 NZ_CP071871:998523-998999 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGAATTAACACAACCCGAATTTGCCCGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGACTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTTTTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG

Structures

Toxin

• AlphaFold DB: A0A075MDK3