Detailed information of TA system TA162594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4429349..4430937
• Replicon: chromosome
• Accession: NZ_CP056077
• Organism: Escherichia coli strain MS1170

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HED12_RS21715
• Protein ID: WP_096964021.1
• Coordinates: 4429615..4430937 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: HED12_RS21710
• Protein ID: WP_001304368.1
• Coordinates: 4429349..4429615 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HED12_RS21695	4425717..4427168	+	1452	WP_000854637.1	tagaturonate reductase	-
HED12_RS21700	4427365..4428279	+	915	WP_001313828.1	bestrophin family inner membrane protein	-
HED12_RS21705	4428283..4429041	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
HED12_RS21710	4429349..4429615	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HED12_RS21715	4429615..4430937	+	1323	WP_096964021.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HED12_RS21720	4431209..4431475	+	267	Protein_4089	helix-turn-helix transcriptional regulator	-
HED12_RS21725	4431908..4432471	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
HED12_RS21730	4432833..4433543	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49313.06 Da
• Isoelectric Point: 8.6972

>T162594 WP_096964021.1 NZ_CP056077:4429615-4430937 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFLVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T162594 NZ_CP071870:1001920-1002201 [Propioniciclava sp. MC1595]
GTGATCGTCTCCTTTGCCGACCGCGATGCCGAACGCCTGTTCCTGCGACAGCGCGTCAAGCGTCTCGACTCTCGTCTGCA
CAAGAAGGCGCTCACAAAGCTGTTGCTACTCGATGCTGCCCACGAGCTTGATGAACTCCGCGTTCCACCGGGGAACCGGC
TGGAAGCACTCAAGGGCGACCGGATCGGTCAGCACAGCATCCGAATCAACGACCAGTGGCGGATCTGCTTCGTCTGGACT
CCTGCGGGCCCCAGCGAGGTTGAGATTGTCGACTATCACTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT162594 WP_001304368.1 NZ_CP056077:4429349-4429615 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT162594 NZ_CP071870:1002212-1002508 [Propioniciclava sp. MC1595]
ATGGATGACAAGATTCCCCCGATCCATCCGGGCGAGATCCTCATGGAGGAGTTCCTCGTTCCAATGGGCGTAACTCAGAA
CCGGCTGGCAGTATCCATCGGCGTTCCGCCGCGTCGGATCAACGAGATCGTGCACGGCAAGCGTCGGATCACGGCGGATA
CGGCTCTGCGTCTGGGGCGCTTCTACAGCATGAGTGCGCAGTTCTGGATCAACCTGCAGGTGCGCTATGACCTTGAGGTC
GAGATGGACGCCCTCGGGGACGCGCTGGACAGCATTCAGCCGCTACGGACTGCATGA

Structures

Antitoxin

• AlphaFold DB: V0VAH2