Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3151355..3151938 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HED12_RS15575 Protein ID WP_000254750.1
Coordinates 3151603..3151938 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HED12_RS15570 Protein ID WP_000581937.1
Coordinates 3151355..3151603 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS15560 3147694..3148995 + 1302 WP_202791681.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HED12_RS15565 3149043..3151277 + 2235 WP_000226797.1 GTP diphosphokinase -
HED12_RS15570 3151355..3151603 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HED12_RS15575 3151603..3151938 + 336 WP_000254750.1 endoribonuclease MazF Toxin
HED12_RS15580 3152010..3152801 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HED12_RS15585 3153029..3154666 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HED12_RS15590 3154754..3156052 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T162583 WP_000254750.1 NZ_CP056077:3151603-3151938 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T162583 NZ_CP071861:c3925736-3925633 [Enterobacter cloacae]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT162583 WP_000581937.1 NZ_CP056077:3151355-3151603 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT162583 NZ_CP071861:3925620-3925771 [Enterobacter cloacae]
CTTTCAATCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References